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| Status |
Public on Jun 04, 2016 |
| Title |
ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inbred and hybrid mouse ESC lines carrying different gene knockouts. By using an allele-specific RNA-seq approach, we demonstrate that ZFP57 loss results in derepression of the imprinted allele of multiple genes in the imprinted clusters. We also find marked epigenetic differences between ICRBS and non-ICRBS suggesting that different cis-acting regulatory functions are repressed by ZFP57 at these two classes of target loci. Overall, these data demonstrate that ZFP57 is pivotal to maintain the allele-specific epigenetic modifications of ICRs that in turn are necessary for maintaining the imprinted expression over long distances. At non-ICRBS, ZFP57 inactivation results in acquisition of epigenetic features that are characteristic of poised enhancers, suggesting that another function of ZFP57 in early embryogenesis is to repress cis-acting regulatory elements whose activity is not yet required.
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| Overall design |
Refer to individual Series
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| Web link |
http://genome.ucsc.edu/cgi-bin/hgTracks?hgS_doOtherUser=submit&hgS_otherUserName=bioinformatico&hgS_otherUserSessionName=Riso_et_al_2016
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| Citation(s) |
27257070 |
| |
| Submission date |
Feb 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Cammisa |
| E-mail(s) |
cammisamarco.py@gmail.com
|
| Organization name |
Institute of Genetics and Biophysics "A. Buzzati Traverso" (IGB-ABT)
|
| Street address |
Via Castellino Pietro, 111
|
| City |
Naples |
| State/province |
Naples |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (23)
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| This SuperSeries is composed of the following SubSeries: |
| GSE77438 |
ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells (BS-seq and RRBS) |
| GSE77440 |
ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells (ChIP-seq) |
| GSE77441 |
ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells (RNA-seq) |
|
| Relations |
| BioProject |
PRJNA310342 |
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