|
| Status |
Public on Apr 30, 2016 |
| Title |
Effects of MBTD1 on gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The NuA4/TIP60 acetyltransferase complex is a key regulator of genome expression and stability. Here, we identified MBTD1 as a new stable subunit of the complex and gleaned intriguing insights into TIP60’s function. Harboring a histone reader domain for H4K20me1/2, MBTD1 allows TIP60 to associate with specific gene promoters and to promote the repair of DNA double strand breaks by homologous recombination.
Total RNA was analyzed on Illumina Human HT-12 V4.0 expression beadchip by McGill University and Genome Québec innovation Centre
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| |
|
| Overall design |
CRISPR/Cas9 technology was used to produce MBTD1 knockout cells in U2OS and to perform genome-wide expression analysis on three different clones compared to wild type cells.
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| |
|
| Contributor(s) |
Jacquet K, Paquet ER, Côté J |
| Citation(s) |
27153538 |
| |
| Submission date |
Feb 17, 2016 |
| Last update date |
Mar 06, 2020 |
| Contact name |
Eric R Paquet |
| Organization name |
EPFL
|
| Department |
Life sciences
|
| Lab |
Naef lab
|
| Street address |
Station 15 CH - 1015
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA312379 |
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