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Series GSE78048 Query DataSets for GSE78048
Status Public on Jan 01, 2017
Title Role of an extracytoplasmic function sigma factor, PGN_0319, in hemin utilization by Porphyromonas gingivalis
Platform organism Porphyromonas gingivalis
Sample organism Porphyromonas gingivalis ATCC 33277
Experiment type Expression profiling by array
Summary Extracytoplasmic function (ECF) sigma factors are known to play an important role in the bacterial response to various environmental stresses. Porphyromonas gingivalis, a prominent etiological agent in human periodontitis, possesses six putative ECF sigma factors. So far, information is limited on an ECF sigma factor, PGN_0319. It has been recently reported that the expression of genes encoding some of the ECF sigma factors including PGN_0319 was affected by hemin availability. The aim of this study was to evaluate the role of PGN_0319 in hemin utilization by P. gingivalis. We evaluated the gene expression profile of the PGN_0319 mutant by DNA microarray, and then real-time reverse transcription PCR analysis was performed to assess genes with altered expression levels. The expressions level of hmuY and hmuR, which encode an outer-membrane protein involved in hemin utilization, and cdhR, a transcriptional regulator of hmuYR, were significantly decreased in the PGN_0319 mutant. The transcription of these genes was restored in the PGN_0319 complemented strain. We observed that the PGN_0319 mutant showed reduced growth in log phase compared with wild type under hemin-limiting condition. By electrophoretic mobility shift assay we demonstrated the recombinant PGN_0319 protein bound to the promoter region of hmuY and cdhR. In addition, we observed that PGN_0319 gene was upregulated in response to low cell density. These results demonstrate that PGN_0319 play an important role in the early growth of P. gingivalis, and directly regulates hmuYR and cdhR, thereby plays a role in the mechanisms involved in hemin utilization by P. gingivalis.
 
Overall design Comparison of 2 strains Wild type: Porphyromonas gingivalis ATCC 33277, 2 samples Mutant: Porphyromonas gingivalis PGN_0319 mutant, 2 samples All strains were harvested in mid log phase.
 
Contributor(s) Ota K, Kikuchi Y, Imamura K, Kita D, Yoshikawa K, Saito A, Ishihara K
Citation(s) 27940243
Submission date Feb 18, 2016
Last update date Apr 02, 2017
Contact name Kazuyuki Ishihara
Organization name Tokyo Dental College
Department Microbiolgy
Street address Misakicho 2-1-14
City Chiyoda-ku
State/province Tokyo
ZIP/Postal code 101-0061
Country Japan
 
Platforms (1)
GPL21477 Agilent-063615 P. gingivalis 8x15K v1.0
Samples (4)
GSM2065599 Pg33277_1 Data from Strain ATCC33277
GSM2065600 Pg33277_2 Data from Strain ATCC33277
GSM2065601 PGN-319_1 Data from Strain KDP315
Relations
BioProject PRJNA312444

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78048_RAW.tar 8.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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