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Status |
Public on Jan 01, 2017 |
Title |
Role of an extracytoplasmic function sigma factor, PGN_0319, in hemin utilization by Porphyromonas gingivalis |
Platform organism |
Porphyromonas gingivalis |
Sample organism |
Porphyromonas gingivalis ATCC 33277 |
Experiment type |
Expression profiling by array
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Summary |
Extracytoplasmic function (ECF) sigma factors are known to play an important role in the bacterial response to various environmental stresses. Porphyromonas gingivalis, a prominent etiological agent in human periodontitis, possesses six putative ECF sigma factors. So far, information is limited on an ECF sigma factor, PGN_0319. It has been recently reported that the expression of genes encoding some of the ECF sigma factors including PGN_0319 was affected by hemin availability. The aim of this study was to evaluate the role of PGN_0319 in hemin utilization by P. gingivalis. We evaluated the gene expression profile of the PGN_0319 mutant by DNA microarray, and then real-time reverse transcription PCR analysis was performed to assess genes with altered expression levels. The expressions level of hmuY and hmuR, which encode an outer-membrane protein involved in hemin utilization, and cdhR, a transcriptional regulator of hmuYR, were significantly decreased in the PGN_0319 mutant. The transcription of these genes was restored in the PGN_0319 complemented strain. We observed that the PGN_0319 mutant showed reduced growth in log phase compared with wild type under hemin-limiting condition. By electrophoretic mobility shift assay we demonstrated the recombinant PGN_0319 protein bound to the promoter region of hmuY and cdhR. In addition, we observed that PGN_0319 gene was upregulated in response to low cell density. These results demonstrate that PGN_0319 play an important role in the early growth of P. gingivalis, and directly regulates hmuYR and cdhR, thereby plays a role in the mechanisms involved in hemin utilization by P. gingivalis.
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Overall design |
Comparison of 2 strains Wild type: Porphyromonas gingivalis ATCC 33277, 2 samples Mutant: Porphyromonas gingivalis PGN_0319 mutant, 2 samples All strains were harvested in mid log phase.
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Contributor(s) |
Ota K, Kikuchi Y, Imamura K, Kita D, Yoshikawa K, Saito A, Ishihara K |
Citation(s) |
27940243 |
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Submission date |
Feb 18, 2016 |
Last update date |
Apr 02, 2017 |
Contact name |
Kazuyuki Ishihara |
Organization name |
Tokyo Dental College
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Department |
Microbiolgy
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Street address |
Misakicho 2-1-14
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City |
Chiyoda-ku |
State/province |
Tokyo |
ZIP/Postal code |
101-0061 |
Country |
Japan |
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Platforms (1) |
GPL21477 |
Agilent-063615 P. gingivalis 8x15K v1.0 |
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Samples (4)
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Relations |
BioProject |
PRJNA312444 |
Supplementary file |
Size |
Download |
File type/resource |
GSE78048_RAW.tar |
8.8 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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