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Series GSE7812 Query DataSets for GSE7812
Status Public on May 17, 2007
Title Effects of aneuploidy on cellular physiology and cell division in haploid yeast.
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Genome variation profiling by array
Genome variation profiling by genome tiling array
Summary Aneuploidy is a condition frequently found in tumor cells but how it affects cellular physiology is not known. We have characterized one aspect of aneuploidy, the gain of extra chromosomes. We created a collection of haploid yeast strains that each bear an extra copy of one or more of almost all of the yeast chromosomes. Their characterization revealed that aneuploid strains share a number of phenotypes, including defects in cell cycle progression, increased glucose uptake and increased sensitivity to conditions interfering with protein synthesis and protein folding. These phenotypes were observed only in strains carrying additional yeast genes indicating that they reflect the consequences of additional transcription and translation as well as the resulting imbalances in cellular protein composition. We conclude that aneuploidy causes not only a proliferative disadvantage but also a set of phenotypes that is independent of the identity of the individual extra chromosomes.
Keywords: CGH, gene expression
Overall design This series of microarrays compares yeast strains carrying extra chromosomes to wt yeast with normal chromosome content. Both DNA/CGH and gene expression comparisons were done, as noted. In some experiments, biological replicates were performed as noted. Reference wt nucleic acid was most commonly labeled with Cy3. Experiments labeled as "swap" have the wt reference nucleic acid labeled with Cy5, and ratio values for these experiments are reported as Cy3/Cy5.
Contributor(s) Torres EM, Sokolsky T, Tucker CM, Chan LY, Boselli M, Dunham MJ, Amon A
Citation(s) 17702937
Submission date May 15, 2007
Last update date Sep 07, 2013
Contact name Maitreya J. Dunham
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
Platforms (2)
GPL884 Agilent-011447 Yeast Oligo Microarray G4140A (Feature Number version)
GPL2883 Agilent-013384 Yeast Oligo Microarray (V2) G4140B (Feature Number version)
Samples (112)
GSM189484 Fig 1AB: Dis I (A6863) batch RNA 1
GSM189485 Fig 1AB: Dis II (A6865) batch RNA 1
GSM189486 Fig 1AB: Dis II (A6865) batch RNA 2
BioProject PRJNA100035

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GSE7812_RAW.tar 275.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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