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| Status |
Public on Dec 16, 2008 |
| Title |
Comparison between longissimus dorsi muscle tissue of Fat and Lean Type Pig, among developmental phases |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was to identify key genes associated with porcine muscle growth and adipose metabolism which different expression in porcine longissimus dorsi muscle tissue between Fat Type Pig(Taihu pig) and Lean Type Pig (Landrace), among developmental phases(Month 0,1,2,3,4,5). The gene expression analyses will increase understanding of impact factors of pork quality by identifying key genes and pathways controlling longissimus dorsi muscle development. Relative real-time RT-PCR was used to confirm differential expression of 5 different expression genes(PPARGC1A, RYR1, IGF2, IGF1R and IGFBP5) which normalized by 3 housekeep genes(ACTB, TBP and TOP2B).
Keywords: time course and breed comparison
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| Overall design |
The different expression experiments between Taihu pig and Landrace used partnership design ( Landrace Month 0 vs Taihu Month 0, 1 vs 1,2 vs 2,3 vs 3, 4 vs 4,5 vs 5).
The different expression experiments among developmental phases(Month 0,1,2,3,4,5) used indirect loop design(Landrace Month 1 vs 0, 2 vs 1,3 vs 2,4 vs 3,5 vs 4,0 vs 5; Taihu Pig Month 1 vs 0, 2 vs 1,3 vs 2,4 vs 3,5 vs 4,0 vs 5).
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| Contributor(s) |
Li M, Li X, Zhu L, Guo Y, Chen L, Li Q, Shuai S, Jiang A, Yang G, Tang G, Jiang Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 21, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Mingzhou Li |
| E-mail(s) |
mingzhou.li@163.com
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| Phone |
86-835-2886000
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| Organization name |
Sichuan Agricultural University
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| Department |
College of Animal Science and Technology
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| Lab |
Institute of Animal Genetics and Breeding
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| Street address |
No.46 Xinkang Road
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| City |
Ya'an |
| State/province |
Sichuan |
| ZIP/Postal code |
625014 |
| Country |
China |
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| Platforms (1) |
| GPL5171 |
Pork Quality Operon 70-mer oligo array |
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| Samples (18)
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| GSM190582 |
Landrace_longissimus dorsi muscle_ Month 0 vs 5 |
| GSM190583 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 0 vs 0 |
| GSM190584 |
Landrace_longissimus dorsi muscle_ Month 1 vs 0 |
| GSM190585 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 1 vs 1 |
| GSM190586 |
Landrace_longissimus dorsi muscle_ Month 2 vs 1 |
| GSM190587 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 2 vs 2 |
| GSM190588 |
Landrace_longissimus dorsi muscle_ Month 3 vs 2 |
| GSM190589 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 3 vs 3 |
| GSM190590 |
Landrace_longissimus dorsi muscle_ Month 4 vs 3 |
| GSM190591 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 4 vs 4 |
| GSM190592 |
Landrace_longissimus dorsi muscle_ Month 5 vs 4 |
| GSM190593 |
Landrace vs Taihu Pig_longissimus dorsi muscle_ Month 5 vs 5 |
| GSM190594 |
Taihu Pig_longissimus dorsi muscle_ Month 0 vs 5 |
| GSM190595 |
Taihu Pig_longissimus dorsi muscle_ Month 1 vs 0 |
| GSM190596 |
Taihu Pig_longissimus dorsi muscle_ Month 2 vs 1 |
| GSM190597 |
Taihu Pig_longissimus dorsi muscle_ Month 3 vs 2 |
| GSM190598 |
Taihu Pig_longissimus dorsi muscle_ Month 4 vs 3 |
| GSM190599 |
Taihu Pig_longissimus dorsi muscle_ Month 5 vs 4 |
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| This SubSeries is part of SuperSeries: |
| GSE7901 |
Swine Profiling of longissimus dorsi muscle and backfat |
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| Relations |
| BioProject |
PRJNA105495 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7857_RAW.tar |
14.2 Mb |
(http)(custom) |
TAR (of TIFF, TXT) |
| Processed data included within Sample table |
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