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Series GSE78689 Query DataSets for GSE78689
Status Public on Feb 11, 2019
Title K13 580Y allele not linked to increased P. falciparum recrudescences in artesunate-treated Aotus
Organism Plasmodium falciparum
Experiment type SNP genotyping by SNP array
Summary Concerns about impending failure of artemisinin compounds (ART) have grown with global use of ART-based combination therapy (ACT) against malaria. WHO has defined Plasmodium falciparum resistance to ART as prolonged parasite clearance half-life in vivo (t1/2) plus the presence of certain K13 Kelch-propeller substitutions, e.g. C580Y. Recrudescences and fever clearance times after ART monotherapy, however, have not correlated well with these criteria. We have crossed K13 C580 wild-type and 580Y-mutant parasites for ART studies in Aotus. Artesunate treated C580- but not 580Y-infections recrudesced requiring retreatment, and K13 type had little or no effect on t1/2. These results challenge K13 and t1/2 variations as markers of increased resistance to ART per se and emphasize the need for effective partner drugs in ACTs.
 
Overall design 57 samples, no replicates, Plasmodium falciparum various clones.
 
Contributor(s) Sá JM, Kaslow SR, Krause MA, Melendez-Muniz V, Salzman RE, Kite WA, Zhang M, Moraes Barros RR, Mu J, Han PK, Mershon JP, Figan CE, Caleon RL, Gibson TJ, Amaratunga C, Engels T, Velmurugan S, Ricklefs S, Straimer J, Gnädig N, Deng B, Liu A, Diouf A, Miura K, Tullo GS, Eastman RT, Chakravarty S, James ER, Udenze K, Li S, Sturdevant DE, Gwadz RW, Porcella SF, Long CA, Fidock DA, Thomas ML, Fay MP, Kim Lee Sim B, Hoffman SL, Adams JH, Fairhurst RM, Su X, Wellems TE
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Submission date Feb 25, 2016
Last update date Feb 13, 2019
Contact name Dan Sturdevant
E-mail(s) dsturdevant@niaid.nih.gov
Phone 4063639248
Organization name NIH
Department NIAID
Lab RTS
Street address 903 S 4th street
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platforms (1)
GPL21519 [Pf_SNP_2_geno_pm] Affymetrix Plasmodium falciparum 75K SNP
Samples (57)
GSM2072646 Plasmodium falciparum clone 803; 803
GSM2072647 Plasmodium falciparum clone GB4; GB4
GSM2072648 Plasmodium falciparum clone 11H5; 36F11 isogenic progeny
Relations
BioProject PRJNA313217

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78689_RAW.tar 1.4 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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