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Series GSE78828 Query DataSets for GSE78828
Status Public on Jul 01, 2016
Title Rhodococcus jostii RHA1 TadA-homolog deletion mutants accumulate less polyhydroxyalkanoates (PHAs) that the parental strain
Organism Rhodococcus jostii RHA1
Experiment type Expression profiling by array
Summary Transcriptome studies confirm the nitrogen limited physiological state of both the wild-type and mutant cells. In addition, multiple differentially expressed genes involved in the synthesis and consumption of pools of acetyl-CoA, acetoacetyl-CoA and 3-hydroxybutyryl-CoA, key metabolites for PHA and TAG synthesis, were identified.
An enrichment analysis of differentially expressed genes in the nitrogen starved wild-type versus the isogenic RHA1_ro02104 mutant strain identified genes in the mutant involved in fatty acid and lipid as well as genes involved in acyl-CoA hydrolysis and triacylglycerol degradation.
 
Overall design An 8 x 15K array study using total RNA recovered from triplicate cultures of Rhodococcus jostii RHA1 under nitrogen rich and nitrogen starved conditions and triplicate cultures of Rhodococcus jostii RHA1 TadA-homolog deletion mutants (2104) under nitrogen rich and nitrogen starved conditions.
 
Contributor(s) Indest KJ, Eberly JO, Hancock DE, Jung CM, Carr MR, Blakeney GA
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Submission date Mar 02, 2016
Last update date Jul 03, 2016
Contact name Dawn E Hancock
E-mail(s) dawn.hancock@usace.army.mil
Phone 6016343711
Organization name DEPARTMENT OF DEFENSE Army Corps of Engineers
Department Engineer Research and Development Center
Lab Environmental Lab
Street address 3909 Halls Ferry Road
City Vicksburg
State/province Mississippi
ZIP/Postal code 39180
Country USA
 
Platforms (1)
GPL21549 Agilent-077006 Rhodococcus jostii RHA1 8x15K custom expression array (Systematic Name version)
Samples (12)
GSM2078313 rha1_ro02104 with N rep1
GSM2078314 rha1_ro02104 with N rep2
GSM2078315 rha1_ro02104 with N rep3
Relations
BioProject PRJNA314082

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78828_RAW.tar 10.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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