|
Status |
Public on Jul 01, 2016 |
Title |
Rhodococcus jostii RHA1 TadA-homolog deletion mutants accumulate less polyhydroxyalkanoates (PHAs) that the parental strain |
Organism |
Rhodococcus jostii RHA1 |
Experiment type |
Expression profiling by array
|
Summary |
Transcriptome studies confirm the nitrogen limited physiological state of both the wild-type and mutant cells. In addition, multiple differentially expressed genes involved in the synthesis and consumption of pools of acetyl-CoA, acetoacetyl-CoA and 3-hydroxybutyryl-CoA, key metabolites for PHA and TAG synthesis, were identified. An enrichment analysis of differentially expressed genes in the nitrogen starved wild-type versus the isogenic RHA1_ro02104 mutant strain identified genes in the mutant involved in fatty acid and lipid as well as genes involved in acyl-CoA hydrolysis and triacylglycerol degradation.
|
|
|
Overall design |
An 8 x 15K array study using total RNA recovered from triplicate cultures of Rhodococcus jostii RHA1 under nitrogen rich and nitrogen starved conditions and triplicate cultures of Rhodococcus jostii RHA1 TadA-homolog deletion mutants (2104) under nitrogen rich and nitrogen starved conditions.
|
|
|
Contributor(s) |
Indest KJ, Eberly JO, Hancock DE, Jung CM, Carr MR, Blakeney GA |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Mar 02, 2016 |
Last update date |
Jul 03, 2016 |
Contact name |
Dawn E Hancock |
E-mail(s) |
dawn.hancock@usace.army.mil
|
Phone |
6016343711
|
Organization name |
DEPARTMENT OF DEFENSE Army Corps of Engineers
|
Department |
Engineer Research and Development Center
|
Lab |
Environmental Lab
|
Street address |
3909 Halls Ferry Road
|
City |
Vicksburg |
State/province |
Mississippi |
ZIP/Postal code |
39180 |
Country |
USA |
|
|
Platforms (1) |
GPL21549 |
Agilent-077006 Rhodococcus jostii RHA1 8x15K custom expression array (Systematic Name version) |
|
Samples (12)
|
|
Relations |
BioProject |
PRJNA314082 |