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| Status |
Public on May 12, 2016 |
| Title |
Comparison of gene expression in M. tuberculosis TrxB2-DUC mutant treated or not with atc and DTT |
| Organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by array
|
| Summary |
M. tuberculosis thioredoxin reductase (TrxB2) is essential for bacterial survival in vitro and in vivo. To gain insight into the pathways dependent on TrxB2, we compared the mRNA profiles of TrxB2-DUC mutant in the presence and absence of atc and DTT.
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| Overall design |
We grew bacteria in 7H9 medium to an OD of 0.5~0.6. We then added atc to a final concentration of 800 ng/ml and DTT to a final concentration of 2 mM. Samples were collected 24 hr after treatment.
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| Contributor(s) |
Lin K, Ehrt S |
| Citation(s) |
27249779 |
| Submission date |
Mar 04, 2016 |
| Last update date |
Jun 02, 2016 |
| Contact name |
Kan Lin |
| E-mail(s) |
kal2025@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Microbiology and Immunology
|
| Lab |
Dr. Sabine Ehrt
|
| Street address |
413 East 69th Street
|
| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL16177 |
Agilent-019943 Genotypic Technology custom M tuberculosis h37Rv Whole Genome 8x15k GE Microarray [Gene Name version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA314325 |
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