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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 16, 2016 |
| Title |
The dynamic translatome of retinal ganglion cell axons during assembly and maintenance of the mouse visual system |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Local mRNA translation mediates the adaptive responses of axons to extrinsic signals but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion cell axons of the developing and adult retinotectal projection in vivo. The embryonic-to-postnatal axonal translatome comprises an evolving subset of enriched genes with axon-specific roles suggesting distinct steps in axon wiring, such as elongation, pruning and synaptogenesis. Adult axons, remarkably, have a complex translatome with strong links to axon survival, neurotransmission and neurodegenerative disease. Translationally co-regulated mRNA subsets share common upstream regulators, and novel sequence elements generated by alternative splicing that promote axonal mRNA translation. Our results indicate that intricate regulation of compartment-specific mRNA translation in mammalian CNS axons supports the formation and maintenance of neural circuits in vivo.
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| Overall design |
The profiling of ribosome-bound mRNAs in mouse retinal ganglion cell axons at 4 different developmental stages
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| Contributor(s) |
Shigeoka T, Jung J, Turner-Bridger B, Ok J, Lin JQ, Amieux PS, Jung H, Holt CE |
| Citation(s) |
27321671, 31825839 |
| Submission date |
Mar 17, 2016 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Christine E Holt |
| Organization name |
University of Cambridge
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| Department |
Department of PDN
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| Street address |
Anatomy Bldg., Downing St.
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3DY |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA315830 |
| SRA |
SRP072106 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79352_RAW.tar |
126.4 Mb |
(http)(custom) |
TAR (of TXT, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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