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| Status |
Public on Apr 09, 2016 |
| Title |
The SET Domain Proteins SUVH2 and SUVH9 Are Required for Pol V Occupancy at RNA-Directed DNA Methylation Loci |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Small RNA-induced transcriptional silencing at transposable elements and other DNA repeats is an evolutionarily conserved mechanism in plants, fungi, and animals. In Arabidopsis thaliana, an RNA-directed DNA methylation pathway is involved in transcriptional silencing. Noncoding RNAs produced by the plant-specific DNA-dependent RNA polymerase V are required for RNA-directed DNA methylation. A chromatin-remodeling complex was previously demonstrated to be required for the occupancy of DNA-dependent RNA polymerase V at RNA-directed DNA methylation loci.
Our results suggest that two putative histone methyltransferases are inactive in their enzymatic activity and act as adaptor proteins to facilitate the recruitment of DNA-dependent RNA polymerase V to chromatin by associating with the chromatin-remodeling complex. In combination with previous studies, we propose that the inactive histone methyltransferases bind to methylated DNA, thereby linking DNA methylation to Pol V transcription at RNA-directed DNA methylation loci.
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| Overall design |
Compare the mRNA profiles of 2-week-old seedlings materials of mutants (nrpe1, suvh2suvh9 and dms3) to wild type Col by Illumina suquencing;
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| Contributor(s) |
He XJ, Liu ZW |
| Citation(s) |
24465213 |
| |
| Submission date |
Apr 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yongqiang Li |
| E-mail(s) |
liyq601@gmail.com
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| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
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| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA317738 |
| SRA |
SRP073025 |
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