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Series GSE8013 Query DataSets for GSE8013
Status Public on Feb 25, 2009
Title Gene expression profiling under low dose and short incubation time of cadmium in primary rat hepatocyte in culture
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The study was performed using primary rat hepatocyte in culture from 4 adult male Sprague-Dawley rats to investigate the changes in gene expression under low dose (4μM) and short exposure (3hrs) of cadmium chloride. By comparing the gene expression profiles of control and cadmium-treated cells, the most dramatic and significant changes were for those genes associated with transcriptional regulation, antioxidant response and control of protein integrity. Changes in other genes involved in cellular physiological responses such as inflammation, growth and apoptosis were also observed. Results were further confirmed by quantitative real time polymerase chain reaction (qRT-PCR).
Keywords: early protective responses
Overall design Primary hepatocytes from four rats were isolated using the collagenase-hyaluronidase perfusion and digestion method. The cells of greater than 85% viability were used in this study. The cells were plated at 90% confluency on culture dishes coated with collagen and maintained in Waymouth's medium containing BSA for 4hrs prior to an overnight incubation with BSA free Waymouth's medium. Control samples and cadmium-treated samples from individual rats were hybridized to RAE 230A arrays (n=4 and 8 microarrays); data were generated and analyzed.
Contributor(s) Hsiao CJ, Stapleton SR
Citation(s) 19010381
Submission date Jun 04, 2007
Last update date Mar 03, 2017
Contact name Susan R Stapleton
Phone 269-387-2853
Fax 269-387-2909
Organization name Western Michigan University
Department Chemistry
Street address 3425 Wood Hall
City Kalamazoo
State/province MI
ZIP/Postal code 49008
Country USA
Platforms (1)
GPL341 [RAE230A] Affymetrix Rat Expression 230A Array
Samples (8)
GSM197832 Primary hepatocyte control, biological rep 1
GSM197833 Primary hepatocyte control, biological rep 2
GSM197834 Primary hepatocyte control, biological rep 3
BioProject PRJNA100809

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Supplementary file Size Download File type/resource
GSE8013_RAW.tar 20.6 Mb (http)(custom) TAR (of CEL, EXP)
Processed data included within Sample table

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