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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 29, 2008 |
| Title |
Prospective isolation of functionally distinct radial glial subtypes - lineage and transcriptome analysis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of
these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.
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| Overall design |
Comparison of radial glial subtypes
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| Contributor(s) |
Pinto L, Mader MT, Irmler M, Gentilini M, Santoni F, Bulfone A, Malatesta P, Beckers J, Götz M, Drechsel D, Blum R, Stahl R |
| Citation(s) |
18372191 |
| Submission date |
Jun 06, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
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| Organization name |
Helmholtz Zentrum Muenchen
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| Department |
Institute of Experimental Genetics
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| Street address |
Ingolstaedter Landstr. 1
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| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (17)
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| GSM198357 |
E18 cortex GFP+ Prominin+ sample S3 |
| GSM198361 |
E18 cortex GFP+ Prominin+ cells, sample S2 |
| GSM198362 |
E18 cortex GFP+ Prominin+ cells, sample S4 |
| GSM198363 |
E18 cortex GFP+ Prominin+ cells, sample S5 |
| GSM200043 |
E14 cortex GFPlow Prominin+ cells, sample S1 |
| GSM200044 |
E14 cortex GFPlow Prominin+ cells, sample S2 |
| GSM200045 |
E14 cortex GFPlow Prominin+ cells, sample S3 |
| GSM200046 |
E14 cortex GFPhigh Prominin+ cells, sample S1 |
| GSM200056 |
E14 cortex GFPhigh Prominin+ cells, sample S2 |
| GSM200057 |
E14 cortex GFPhigh Prominin+ cells, sample S3 |
| GSM200058 |
E14 cortex GFPhigh Prominin+ cells, sample S4 |
| GSM200060 |
E14 cortex GFPhigh cells sample 1 |
| GSM200061 |
E14 cortex GFPlow cells sample 1 |
| GSM200578 |
E14 cortex GFPhigh cells sample 2 |
| GSM200581 |
E14 cortex GFPlow cells sample 2 |
| GSM200582 |
E14 cortex GFPhigh cells sample 3 |
| GSM200583 |
E14 cortex GFPlow cells sample 3 |
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| Relations |
| BioProject |
PRJNA100847 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8034_RAW.tar |
58.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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