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| Status |
Public on Apr 20, 2016 |
| Title |
Mincle-mediated signaling leads to characteristic gene expressions in rat mast cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Rat mast cell line RBL-2H3 was analyzed to investigate the molecular mechanism of Mincle-mediated activation and responses of mast cells.
Mincle-mediated signaling stimulated characteristic gene expression, such as IL-3, IL-4, IL-9, IL-13, IL-31 and TNFα.
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| Overall design |
RBL-2H3 cell line stably expressing Mincle was established. Non-stimulated cells (Control) compared to Mincle-stimulated cells (Myc) in order to comfirm Mincle-mediated signaling-inducible genes. Mincle-stimulated cells compared to Syk inhibitor R406-pretreated cells (R406) in order to examine the importance of Syk for Mincle-mediated gene up-regulations.
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| Contributor(s) |
Honjoh C, Chihara K, Yoshiki H, Yamauchi S, Takeuchi K, Kato Y, Hida Y, Iwano M, Ishizuka T, Sada K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 19, 2016 |
| Last update date |
Apr 23, 2016 |
| Contact name |
Chisato Honjoh |
| E-mail(s) |
honjo@u-fukui.ac.jp
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| Organization name |
University of Fukui
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| Street address |
23-3 Matsuoka-Shimoaizuki
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| City |
Eiheiji |
| State/province |
Fukui |
| ZIP/Postal code |
910-1193 |
| Country |
Japan |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA318859 |
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