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Series GSE80531 Query DataSets for GSE80531
Status Public on Jul 01, 2016
Title Transcriptomic and phenotypic analysis reveals new functions for the Tat pathway in Yersinia pseudotuberculosis [dtatC-vs-ip_37C-stat]
Platform organism Yersinia pseudotuberculosis
Sample organism Yersinia pseudotuberculosis IP 32953
Experiment type Expression profiling by array
Summary The Twin-arginine translocation (Tat) system promotes secretion of folded proteins that in bacteria are identified via an N-terminal signal peptide. Tat systems are associated with virulence in many bacterial pathogens and our previous studies revealed that Tat deficient Yersinia pseudotuberculosis was severely attenuated for virulence. However, in silico predictions did not reveal any obvious virulence factors among the potential Tat substrates encoded by Y. pseudotuberculosis. Aiming to identify Tat dependent pathways and phenotypes of relevance for in vivo infection, we analysed the global transcriptome of parental and ∆tatC mutant strains of Y. pseudotuberculosis during exponential and stationary growth at 26°C and 37°C. The most significant changes in the transcriptome of the ∆tatC mutant were seen at 26°C during stationary phase growth and these included the altered expression of genes related to virulence, stress responses and metabolism. Subsequent phenotypic analysis based on these transcriptome changes revealed several novel Tat dependent phenotypes including decreased YadA expression, impaired growth under iron-limiting and high copper concentrations as well as sensitivity to acidic pH and SDS. Several functionally related Tat substrates were also verified to contribute to these phenotypes. Interestingly, the phenotypes of the Tat deficient strain were generally more pronounced than for the individual mutants of the genes encoding the specific Tat substrates. Altogether, this provides new insight into the impact of Tat deficiency on in vivo fitness and survival/replication of Y. pseudotuberculosis during infection.
 
Overall design Y. pseudotuberculosis IP32953 was grown in LB medium supplemented with 2.5 mM CaCl2 under aeration for one hour at 26°C and then shifted to 37°C. Total RNA was extracted using SV Total RNA Isolation System (Promega). The samples were treated with RNase-free DNase (Roche Applied Science) and the quality of the RNA was confirmed by the lack of PCR amplification of the hns gene and by using an Agilent 2100 Bioanalyzer.
 
Contributor(s) Avican U, Heroven AK, Beckstette M
Citation(s) 27501981
Submission date Apr 21, 2016
Last update date Sep 30, 2016
Contact name Ann Kathrin Heroven
E-mail(s) Annkathrin.Heroven@helmholtz-hzi.de
Phone +49-(0)53161815705
Organization name Helmholtz Centre for Infection Research
Department Molecular Infection Biology
Street address Inhoffenstr. 7
City Braunschweig
State/province Niedersachsen
ZIP/Postal code 38124
Country Germany
 
Platforms (1)
GPL15095 Agilent-020412 Yersinia pseudotuberculosis YPIII gene expression array
Samples (4)
GSM2129874 dtatC-vs-ip_37C-stat_GEO_sample01
GSM2129875 dtatC-vs-ip_37C-stat_GEO_sample02
GSM2129876 dtatC-vs-ip_37C-stat_GEO_sample03
This SubSeries is part of SuperSeries:
GSE80532 Transcriptomic and phenotypic analysis reveals new functions for the Tat pathway in Yersinia pseudotuberculosis
Relations
BioProject PRJNA319201

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80531_RAW.tar 6.0 Mb (http)(custom) TAR (of TAB, TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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