NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE80654 Query DataSets for GSE80654
Status Public on Sep 19, 2017
Title FACS sorting of human adipose tissue stromal vascular fraction
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Adipose tissue from 6 non-obese patients was collagenase treated and adipocytes separated from the stromal vascular fraction(SVF). SVF was then FACS sorted for the following fractions CD45-/CD34+/CD31+ (endothelial), CD45-/CD34+/CD31- (progenitor), CD45+/CD14+ (monocyte/macrophage), CD45+/CD14-(Leukocyte). RNA was isolated from adipocyte, SVF, progenitor, macrophage/monocyte and leukocyte fractions and analyzed on the Affymetrix Human Transcriptome 2.0 array. We also sorted SVF from an additional 13 (10 non-obese, 9 obese) patients and sent progenitor RNA for Affymetrix Human Transcriptome 2.0 array analysis.
 
Overall design Adipose tissue from 6 non-obese patients was collagenase treated and adipocytes separated from the stromal vascular fraction(SVF). SVF was then FACS sorted for the following fractions CD45-/CD34+/CD31+ (endothelial), CD45-/CD34+/CD31- (progenitor), CD45+/CD14+ (monocyte/macrophage), CD45+/CD14-(Leukocyte). RNA was isolated from adipocyte, SVF, progenitor, macrophage/monocyte and leukocyte fractions and analyzed on the Affymetrix Human Transcriptome 2.0 array. We also sorted SVF from an additional 13 (10 non-obese, 9 obese) patients and sent progenitor RNA for Affymetrix Human Transcriptome 2.0 array analysis.
 
Contributor(s) Ehrlund A, Laurencikiene J
Citation(s) 29087381
Submission date Apr 25, 2016
Last update date Jul 25, 2021
Contact name Jurga Laurencikiene
Organization name Karolinska Institutet
Street address Hälsovägen 7
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (51)
GSM2132158 CD45-/CD34+/CD31- cells from non-obese human adipose SVF JL01_P6_1
GSM2132159 CD45-/CD34+/CD31- cells from non-obese human adipose SVF JL02_P6_2
GSM2132160 CD45-/CD34+/CD31- cells from non-obese human adipose SVF JL03_P6_3
Relations
BioProject PRJNA319629

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80654_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap