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| Status |
Public on Sep 14, 2016 |
| Title |
Silencing of cryptic prophages in Corynebacterium glutamicum |
| Platform organisms |
Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168 |
| Sample organism |
Corynebacterium glutamicum |
| Experiment type |
Expression profiling by array
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| Summary |
DNA microarrays were applied to investigate the transcriptome of C. glutamicum cells and to identify potential target genes under CgpS countersilencing conditions. The silencing activity of CgpS was reduced by overexpression of the N-terminal part, which was placed under the control of the inducible tac promoter in the pAN6 plasmid. The expression of the N terminal part was induced with 100 µM IPTG.
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| Overall design |
DNA microarray analysis were performed with three biological replicates. The transcriptome of C. glutamicum cells in which CgpS activity was downregulated by countersilencing was compared to the transcriptome of C. glutamicum wt cells containing the empty plasmid. Countersilencing was achieved by overexpression of the N-terminal part of CgpS with 100 µM IPTG.
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| Contributor(s) |
Pfeifer E, Hünnefeld M, Popa O, Baumgart M, Polen T, Kohlheyer D, Frunzke J |
| Citation(s) |
27492287 |
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| Submission date |
Apr 26, 2016 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Tino Polen |
| E-mail(s) |
t.polen@fz-juelich.de
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| Organization name |
Forschungszentrum Jülich GmbH
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| Department |
IBG-1: Biotechnology
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| Street address |
Leo Brandt Str.
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| City |
Juelich |
| State/province |
NRW |
| ZIP/Postal code |
52425 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA319652 |
