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| Status |
Public on Sep 25, 2017 |
| Title |
Effect of BORIS depletion on transcriptome of breast cancer cell line MCF7 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analysis of BORIS depleted MCF7 cells
To delineate the alternative pre-mRNA splicing program mediated by BORIS in breast cancer cells, BORIS was depleted in MCF7 using shRNA, and the resultant changes in alternative pre-mRNA splicing and mRNA expression pattern was profiled using Affymetrix Human Transcriptome 2.0 Array
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| Overall design |
We analyzed shContol and shBORIS transfected MCF7 cell line using the Affymetrix Human Transcriptome 2.0 platform. Array data was processed by Affymetrix transcriptome console Computational Tool. Biological replicates were performed.
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| Contributor(s) |
Singh S, Narayanan SP, Biswas K, Gupta A, Ahuja N, Yadav S, Panday RK, Atul S, Sharan SK, Shukla S |
| Citation(s) |
29073069 |
| Submission date |
May 02, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Sanjeev Shukla |
| E-mail(s) |
sanjeevs@iiserb.ac.in
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| Phone |
9584635471
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| Organization name |
Indian Institute of Science Education and Research Bhopal
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| Department |
Biological Sciences
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| Lab |
Epigenetics and RNA Processing Lab (ERPL)
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| Street address |
Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh, INDIA
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| City |
Bhopal |
| State/province |
Madhya Prades |
| ZIP/Postal code |
462066 |
| Country |
India |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA320262 |
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