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| Status |
Public on May 11, 2016 |
| Title |
miRNA profiling of mature C2C12 myotubes using modified (COS) vs standard (STD) tissue culture conditions |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
miRNA profiling comparing modified (COS = Cytokine (low dose), Oxygen (physiological), Stimulation (low frequency electrical)) or standard (STD) culture condtions for 72hrs prior to harvest at day 8 post differentiation. Secondary experiment to assess additional impact of dexamethasone when given in COS conditions.
5 arrays were labeled with same RNA in each channel (self-self hybridisations) to establish parameters for detecting real change for each probe on the arrays.
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| |
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| Overall design |
Main experiment: two-conditions; 6 biological replicates; 1 replicate per array. Secondary experiment: two conditions: 2 biological replicates; 1 replicate per array
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| |
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| Contributor(s) |
Jagoe RT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 05, 2016 |
| Last update date |
May 13, 2016 |
| Contact name |
R. Thomas Jagoe |
| E-mail(s) |
thomas.jagoe@mcgill.ca
|
| Phone |
15143408222
|
| Organization name |
McGill University
|
| Department |
Medicine
|
| Street address |
Jewish General Hospital, 3755 Cote Ste Catherine
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
| |
|
| Platforms (3) |
| GPL21849 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno [miRBase 18.0] |
| GPL21850 |
miRCURY LNA microRNA Array, 5th generation - hsa, mmu & rno [miRBase 18.0] |
| GPL21851 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno [miRBase 18.0] |
|
| Samples (13)
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| Relations |
| BioProject |
PRJNA321189 |
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