|
| Status |
Public on Sep 11, 2016 |
| Title |
Utility of circulating serum miRNAs as biomarkers of early cartilage degeneration in animal models of post-traumatic osteoarthritis and inflammatory arthritis |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The purpose of this study was to identify potential serum microRNA (miRNA) biomarkers of cartilage degeneration comparing preclinical mouse models of post-traumatic osteoarthritis and inflammatory arthritis.
|
| |
|
| Overall design |
Cartilage degeneration was induced in one knee of 10-12 week old male C57BL6 mice by surgical destabilization of the medial meniscus (DMM) or intra-articular injection of methylated-bovine-serum-albumin (AIA), with sham-operated or saline-injected control animals (n=6/treatment/time). Total serum RNA and knee joints were isolated at 1, 4 and 16 weeks post-induction. Cartilage degeneration was scored histologically. Serum miRNA expression profiling was performed using Agilent microarrays and validated by quantitative PCR.
|
| |
|
| Contributor(s) |
Kung LH, Zaki S, Ravi V, Rowley L, Bell KM, Bateman JF, Little CB, Smith MM |
| Citation(s) |
27621213 |
| |
| Submission date |
May 05, 2016 |
| Last update date |
Sep 28, 2016 |
| Contact name |
John Bateman |
| Organization name |
Murdoch Childrens Research Institute
|
| Department |
Skeletal Biology
|
| Lab |
John Bateman
|
| Street address |
Flemington Road
|
| City |
Melbourne |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL22486 |
Agilent-046065 Mouse miRNA Microarray (miRBase release 21 miRNA ID version) |
|
| Samples (71)
|
|
| Relations |
| BioProject |
PRJNA320723 |
Less...
More...