|
| Status |
Public on May 12, 2016 |
| Title |
Dam(ID)-profiling in B cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Both chromatin access, and binding of AID in B cells are of major scientific interest. Therefore DamID was setup in B cells. AID specific binding could not be identified.
In contrast to binding, DAM profiling provide a upmost useful tool in defining chromatin access in vivo.
|
| |
|
| Overall design |
Ramos B cells were transduced with Dam-only or with AID-Dam lentivirus. Within the cell, Dam methylates adenines near sites where the protein of interest would interacts (here AID) with the DNA. These methylated sequences are subsequently amplified by a methylation-specific PCR protocol as previsously described by Vogel et al (2007), and identified by hybridization to 2.1 Economy Whole Genome Nimblegen microarrays.
|
| |
|
| Contributor(s) |
Hogenbirk MA, Heideman MR, Brugman W, Jacobs H |
| Citation(s) |
27303044 |
| |
| Submission date |
May 11, 2016 |
| Last update date |
Jul 21, 2016 |
| Contact name |
Marc Hogenbirk |
| Organization name |
Netherlands Cancer Institute
|
| Street address |
Plesmanlaan
|
| City |
Amsterdam |
| ZIP/Postal code |
1066CX |
| Country |
Netherlands |
| |
|
| Platforms (2) |
| GPL16052 |
NimbleGen Human ChIP-chip 2.1M Economy Whole-Genome Tiling - Array 1 of 4 [100718_HG18_Economy_01_HX1] |
| GPL16053 |
NimbleGen Human ChIP-chip 2.1M Economy Whole-Genome Tiling - Array 2 of 4 [100718_HG18_Economy_02_HX1] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA321322 |
Less...
More...