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| Status |
Public on Feb 15, 2017 |
| Title |
APOL1 variant expression in mouse podocytes cause kidney disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Next-generation sequencing (NGS) has become an important tool in molecular charactarization of animal models. APOL1 variants are associated with end stage renal disease in African Americans. We developed a mouse model with podocyte specific over expression of APOL1. Differential molecular signatures were identified between the groups by RNA-Sequencing on kidney.
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| Overall design |
Total RNA of transgenic and control mice kidneys was isolated using RNeasy Mini Kit (Qiagen). RNA quality was assessed with the Agilent Bioanalyzer 2100 and RIN scores above 7 were used for cDNA production. Library was prepared and one microgram total RNA was used to isolate poly(A) purified mRNA using the Illumina TruSeq RNA Preparation Kit. We sequence our sample for single-end 100bp, and the annotated RNA counts (fastq) were calculated by Illumina’s CASAVA 1.8.2.
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| Contributor(s) |
Qiu C, Beckerman P, Park AS, Susztak K |
| Citation(s) |
28218918 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK105821 |
APOL1 associated kidney disease |
UNIVERSITY OF PENNSYLVANIA |
KATALIN SUSZTAK |
| DP3 DK108220 |
Epigenetic drivers and biomarkers of diabetic kidney disease |
UNIVERSITY OF PENNSYLVANIA |
KATALIN SUSZTAK |
| P50 DK064236 |
PILOT--MOLECULAR PATHOLOGY OF DIABETIC NEPHROPATHY |
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY |
KATALIN SUSZTAK |
| R01 DK076077 |
Role of the Notch Pathway in Kidney Injury |
UNIVERSITY OF PENNSYLVANIA |
KATALIN SUSZTAK |
| R01 DK087635 |
Epigenetic Landscape of Chronic Kidney Disease |
UNIVERSITY OF PENNSYLVANIA |
KATALIN SUSZTAK |
| R01 DK105821 |
APOL1 associated kidney disease |
UNIVERSITY OF PENNSYLVANIA |
KATALIN SUSZTAK |
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| |
| Submission date |
May 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Chengxiang Qiu |
| E-mail(s) |
cxqiu@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Jay Shendure
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| Street address |
William H. Foege Hall, 3720 15th Ave NE
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA321754 |
| SRA |
SRP075245 |
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