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| Status |
Public on Oct 12, 2016 |
| Title |
ChIP-seq of HSF-1 and Pol II in C. elegans larval development and heat shock |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To understand the function and regulation of the C. elegans heat shock factor (HSF-1) in larval development, we have used ChIP-seq to analyze the occupancy of HSF1 and RNA Pol II in L2 larvae and young adult (YA) animals grown at 20°C or upon heat shock at 34°C for 30 min. In addition, we have used RNA-seq to analyze the transcriptomes of wild type (N2), hsf-1(ok600) mutants and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 larvae grown at 20°C and characterized the gene expression change by heat shock in wild type (N2) animals at L2 stage.
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| Overall design |
Experiment type: ChIP-seq. Biological Source: strain: AM1061; developmental dtage: L2 Larva, young adult (YA). Experimental Factors: temperature: 20 degree celsius, 34 degree celsius; antibodies: anti-GFP (clontech, #632592), anti-AMA-1 (Novus, #38520002).
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| Contributor(s) |
Morimoto RI, Li J |
| Citation(s) |
27688402 |
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| Submission date |
May 17, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jian Li |
| E-mail(s) |
jian.li@northwestern.edu
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| Organization name |
Northwestern University
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| Department |
Molecular Biosciences
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| Lab |
Morimoto
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| Street address |
2205 Tech Drive, Hogan 2-100
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| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (18)
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| GSM2155056 |
ChIP-seq of HSF-1 in L2 larvae at 20°C, replicate 1 |
| GSM2155057 |
ChIP-seq of HSF-1 in L2 larvae at 20°C, replicate 2 |
| GSM2155058 |
ChIP-seq of HSF-1 in L2 larvae at 34°C, replicate 1 |
| GSM2155059 |
ChIP-seq of HSF-1 in L2 larvae at 34°C, replicate 2 |
| GSM2155060 |
ChIP-seq of Pol II in L2 larvae at 20°C, replicate 1 |
| GSM2155061 |
ChIP-seq of Pol II in L2 larvae at 20°C, replicate 2 |
| GSM2155062 |
ChIP-seq of Pol II in L2 larvae at 34°C, replicate 1 |
| GSM2155063 |
ChIP-seq of Pol II in L2 larvae at 34°C, replicate 2 |
| GSM2155064 |
ChIP-seq of HSF-1 in YA animals at 20°C, replicate 1 |
| GSM2155065 |
ChIP-seq of HSF-1 in YA animals at 20°C, replicate 2 |
| GSM2155066 |
ChIP-seq of HSF-1 in YA animals at 34°C, replicate 1 |
| GSM2155067 |
ChIP-seq of HSF-1 in YA animals at 34°C, replicate 2 |
| GSM2155068 |
ChIP-seq of Pol II in YA animals at 20°C, replicate 1 |
| GSM2155069 |
ChIP-seq of Pol II in YA animals at 20°C, replicate 2 |
| GSM2155070 |
ChIP-seq of Pol II in YA animals at 34°C, replicate 1 |
| GSM2155071 |
ChIP-seq of Pol II in YA animals at 34°C, replicate 2 |
| GSM2155072 |
Input DNA for ChIPs in L2 Larvae |
| GSM2155073 |
Input DNA for ChIPs in YA animals |
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| This SubSeries is part of SuperSeries: |
| GSE81523 |
E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response |
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| Relations |
| BioProject |
PRJNA321852 |
| SRA |
SRP075275 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81521_L2-HS-HSF_combined.bedgraph.gz |
83.5 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_L2-HS-PolII_combined.bedgraph.gz |
46.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_L2-control-HSF_combined.bedgraph.gz |
45.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_L2-control-PolII_combined.bedgraph.gz |
40.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_YA-HS-HSF_combined.bedgraph.gz |
59.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_YA-HS-PolII_combined.bedgraph.gz |
281.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_YA-control-HSF_combined.bedgraph.gz |
87.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE81521_YA-control-PolII_combined.bedgraph.gz |
217.3 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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