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Series GSE81706 Query DataSets for GSE81706
Status Public on Feb 16, 2017
Title Germline- and soma-specific heritable epigenetic silencing at an endogenous locus (small RNA-Seq)
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The emergence and transmission of epigenetic signals across generations can quickly and efficiently alter gene expression in a population. We describe an epigenetic silencing signal whose initiation, transmission properties, genetic requirements and site of action are distinct from previously described epigenetic inheritance in C. elegans. A multi-copy transgene containing the region upstream of sid-1 silences sid-1 and upstream genes. Once established, silencing is stable in the absence of the array and can be maintained without selection for 13 generations. We show that the silenced state can be transmitted to progeny in the absence of the silenced locus, but that inherited silencing is dependent on the nuclear RNAi Argonaute HRDE-1, which stabilizes silencing siRNAs that target sid-1 exons. Notably, at each generation, the RNAi-dependent germline silenced sid-1 locus transitions to a chromatin-dependent silenced state in somatic cells, indicating that the mechanisms of transgenerational silencing in the soma and germline are distinct.
Overall design Small RNA-seq in wild-type (2 replicates), Psid-1::gfp (4 replicated), hrde-1;Psid-1::gfp (2 replicates), non-array silenced F6 (2 replicates) and non-array de-silenced F21 (2 replicates) worms
Contributor(s) Minkina O, Hunter CP
Citation(s) 28212751
Submission date May 20, 2016
Last update date May 15, 2019
Contact name Olga Minkina
Organization name Harvard University
Department Molecular and Cellular Biology
Lab Craig P. Hunter
Street address 16 Divinity Ave.
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (14)
GSM2171652 N2 #1 Small RNA-seq
GSM2171653 HC125 #1 Small RNA-seq
GSM2171654 N2 #2 Small RNA-seq
This SubSeries is part of SuperSeries:
GSE81708 Germline- and soma-specific heritable epigenetic silencing at an endogenous locus
BioProject PRJNA322339
SRA SRP075488

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Supplementary file Size Download File type/resource
GSE81706_Small_RNA_21-26nt_reads_HT-seqCounts.xlsx 1.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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