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| Status |
Public on Feb 09, 2017 |
| Title |
Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by array
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| Summary |
To learn more about the function of Pos19, we constructed an over-expression strain. For this purpose, the Pos19 gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced Pos19 over-expression compared to an empty vector control. Total RNA from the over-expression (pPos19) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate Pos19-induced changes on transcriptome level.
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| Overall design |
RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.
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| Contributor(s) |
Berghoff BA, Klug G |
| Citation(s) |
27669425 |
| |
| Submission date |
May 20, 2016 |
| Last update date |
Feb 11, 2017 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
Gabriele.Klug@mikro.bio.uni-giessen.de
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| Organization name |
Justus-Liebig University Gießen
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| Department |
Molecular- and Microbiology
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| Street address |
Heinrich-Buff-Ring 26-32
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| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL21931 |
Agilent-27061/Klug lab - Rhodobacter sphaeroides - 15k array Version 2 (144 sRNA) (condensed Probe Identifier version) |
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| Samples (2) |
| GSM2173512 |
R. sphaeroides WT 2.4.1_pPos19 versus pBBR after 7 min of singlet oxygen stress_replicate A |
| GSM2173514 |
R. sphaeroides WT 2.4.1_pPos19 versus pBBR after 7 min of singlet oxygen stress_replicate B |
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| Relations |
| BioProject |
PRJNA322353 |
