|
| Status |
Public on Jun 14, 2016 |
| Title |
mRNA cap methylation in pluripotency and differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The mRNA cap recruits factors essential for transcript processing and translation initiation. We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation. Expression of the mRNA cap methyltransferase activating subunit, RAM, is elevated in ESCs resulting in high levels of mRNA cap methylation and expression of Oct4 and Sox2 and other pluripotency-associated factors. During neural differentiation, RAM is suppressed which is required for loss of Oct4 and Sox2 and correct expression of neural markers.
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| |
|
| Overall design |
Cells were treated with control or RAM siRNA and cytosolic RNA or polysome RNA (actively translated) was sequenced.
|
| |
|
| Contributor(s) |
Suska O, Cowling V |
| Citation(s) |
27452456 |
| |
| Submission date |
May 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Victoria Cowling |
| E-mail(s) |
v.h.cowling@dundee.ac.uk
|
| Organization name |
University of Dundee
|
| Department |
School of Life Sciences
|
| Lab |
Cowling lab
|
| Street address |
Dow Street
|
| City |
Dundee |
| ZIP/Postal code |
DD1 5EH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
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|
| Relations |
| BioProject |
PRJNA322696 |
| SRA |
SRP075667 |
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