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| Status |
Public on Aug 25, 2016 |
| Title |
Bulk RNA-seq analysis of Type 5 retinal bipolar cells from the Htr3a-GFP line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
15,000 GFP+ cells were collected from two replicates of the Htr3a GFP line into RNAlater (ThermoFisher, AM7024). RNA was purified and bulk RNA-seq was performed using the Ovation RNA-seq system V2 (Nugen, 7102-32)
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| |
|
| Overall design |
Bulk RNA-seq analysis of Type 5 retinal bipolar cells (2 biological replicates)
|
| |
|
| Contributor(s) |
Shekhar K |
| Citation(s) |
27565351 |
| Submission date |
May 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Karthik Shekhar |
| E-mail(s) |
karthik@broadinstitute.org
|
| Phone |
217-979-9852
|
| Organization name |
Broad Institute
|
| Street address |
415 Main St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE81905 |
Single cell RNA-sequencing of retinal bipolar cells |
|
| Relations |
| BioProject |
PRJNA323372 |
| SRA |
SRP075721 |
