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Status |
Public on Dec 23, 2016 |
Title |
Endoplasmic reticulum as a hub organizing the activities of microRNAs and a subset of endogenous siRNAs in Arabidopsis [RNA-Seq] |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The endoplasmic reticulum (ER) was implicated as the site of microRNA (miRNA)-mediated translation repression in plants. Here, we examined the ER- and rough ER-associated small RNAome, transcriptome and translatome. We found that almost all cellular transcripts were present on membrane-bound polysomes (MBPs), and miRNAs were enriched in membranes and on MBPs. miRNAs were recruited to membranes by their effector protein AGO1, whereas AGO1 associated with membranes and, partly ribosomes, in an RNA-independent manner. Most strikingly, 22 ntmiRNA isoformsand a set of 22 ntsiRNAs, were enriched in the membrane fraction and onMBPs, where they trigger phasedsecondary siRNAproduction from target transcripts. Loss of membrane association of the 22 nt small RNAs resulted in the loss of phasing in secondary siRNA production.These findings point to the ER as a hub that hosts and organizes endogenous small RNAs in plants.
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Overall design |
Examination of mRNA in 4 cell fractions of Col-0 seedlings with 3 biological replicates
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Contributor(s) |
Chen X |
Citation(s) |
27938667 |
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Submission date |
May 30, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Chenjiang You |
E-mail(s) |
chenjiang.you@ucr.edu, youchenjiang2003@hotmail.com
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Organization name |
Fudan University
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Street address |
2005 Songhu Rd
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City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200438 |
Country |
China |
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Platforms (1) |
GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE82041 |
Endoplasmic reticulum as a hub organizing the activities of microRNAs and a subset of endogenous siRNAs in Arabidopsis |
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Relations |
BioProject |
PRJNA323731 |
SRA |
SRP075885 |