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Series GSE82082 Query DataSets for GSE82082
Status Public on Oct 31, 2016
Title A study of Mediator subunit enhancer-promoter binding
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mediator is a transcriptional co-activator recruited to enhancers by DNA-binding activators, and it also interacts with RNA polymerase (Pol) II as part of the preinitiation complex (PIC). We demonstrate that a single Mediator complex associates with the enhancer and core promoter in vivo, indicating that it can physically bridge these transcriptional elements. However, the Mediator kinase module associates strongly with the enhancer, but not with the core promoter, and it dissociates from the enhancer upon depletion of the TFIIH kinase. Severing the kinase module from Mediator by removing the connecting subunit Med13 does not affect Mediator association at the core promoter, but increases occupancy at enhancers. Thus, Mediator undergoes a compositional change in which the kinase module, recruited via Mediator to the enhancer, dissociates from Mediator to permit association with Pol II and the PIC. As such, Mediator acts as a dynamic bridge between the enhancer and core promoter.
 
Overall design 8 Mediator subunits' genome-wide binding is tested by Chip-seq before and after Kin28 anchor-away.
 
Contributor(s) Petrenko N, Jin Y, Wong KH, Struhl K
Citation(s) 27773675
Submission date May 31, 2016
Last update date May 15, 2019
Contact name Natalia Petrenko
Organization name Harvard Medical School
Department BCMP
Lab Struhl lab
Street address 240 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL17143 Illumina MiSeq (Saccharomyces cerevisiae)
Samples (16)
GSM2183225 kin28-AA_minusrap_Rgr1
GSM2183226 kin28-AA_plusrap_Rgr1
GSM2183227 kin28-AA_minusrap_Gal11
Relations
BioProject PRJNA323937
SRA SRP075926

Download family Format
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Supplementary file Size Download File type/resource
GSE82082_RAW.tar 640.7 Mb (http)(custom) TAR (of BIGWIG, SGR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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