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| Status |
Public on Jun 12, 2017 |
| Title |
Grapevine grafting: scion transcript profiling and defense-related metabolites induced by different rootstocks. |
| Organism |
Vitis vinifera |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Genotype, climate, soil qualities, and rootstocks are among the main factors that influence grape development as well as fruit and wine composition. In this work, the rootstock/scion interactions were studied using transcriptomic and metabolic approaches on leaves of Gaglioppo variety, grafted onto thirteen different rootstocks growing in the same locations. The experiments were performed in a vineyard in the south of Italy, characterized by the Mediterranean climate with warm and dry summers. Whole leaf transcriptome of ‘Gaglioppo’ grafted on five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary (e.g. carbohydrate and transport) and secondary metabolism (e.g. phenylpropanoids pathways and response to stress) was observed. Interestingly, genes involved in the priming defense responses (e.g stilbenes and defense genes) were strongly activated in some graft combinations. These results were further confirmed by the quantification of stilbene contents. According with these observations, the leaves of ‘Gaglioppo’ grafted on 1103 Paulsen showing the lowest levels of stilbene synthase transcripts and stilbene metabolites, reveal the greater sensitivity to downy mildew in in vitro assay. This study carrying out an extensive analysis of rootstock effects to scion leaves open a useful way to unravel this complex interaction
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| Overall design |
Differential expression analysis of Gaglioppo variety grafted onto five different rootstocks
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| Contributor(s) |
Chitarra W, Perrone I, Avanzato C, Minio A, Boccacci P, Santini D, Gilardi G, Siciliano I, Gullino ML, Delledonne M, Mannini F, Gambino G |
| Citation(s) |
28496453 |
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| Submission date |
Jun 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrea Minio |
| E-mail(s) |
minio.ing.andrea@gmail.com
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| Organization name |
Università di Verona
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| Department |
Applied Biotechnologies
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| Lab |
Functional Genomics Lab
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| Street address |
Via dell'Artigliere 8
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| City |
Verona |
| ZIP/Postal code |
37129 |
| Country |
Italy |
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| Platforms (1) |
| GPL19359 |
Illumina HiSeq 1000 (Vitis vinifera) |
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| Samples (15)
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| GSM2188939 |
Gaglioppo grafted on 1103 Paulsen, Replicate A |
| GSM2188940 |
Gaglioppo grafted on 1103 Paulsen, Replicate B |
| GSM2188941 |
Gaglioppo grafted on 1103 Paulsen, Replicate C |
| GSM2188942 |
Gaglioppo grafted on Kober 5BB, Replicate A |
| GSM2188943 |
Gaglioppo grafted on Kober 5BB, Replicate B |
| GSM2188944 |
Gaglioppo grafted on Kober 5BB, Replicate C |
| GSM2188945 |
Gaglioppo grafted on 3309 Couderc, Replicate A |
| GSM2188946 |
Gaglioppo grafted on 3309 Couderc, Replicate B |
| GSM2188947 |
Gaglioppo grafted on 3309 Couderc, Replicate C |
| GSM2188948 |
Gaglioppo grafted on 41B, Replicate A |
| GSM2188949 |
Gaglioppo grafted on 41B, Replicate B |
| GSM2188950 |
Gaglioppo grafted on 41B, Replicate C |
| GSM2188951 |
Gaglioppo grafted on 17-37Mgt, Replicate A |
| GSM2188952 |
Gaglioppo grafted on 17-37Mgt, Replicate B |
| GSM2188953 |
Gaglioppo grafted on 17-37Mgt, Replicate C |
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| Relations |
| BioProject |
PRJNA325007 |
| SRA |
SRP076309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE82317_GEO_expression_matrix.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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