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Series GSE82317 Query DataSets for GSE82317
Status Public on Jun 12, 2017
Title Grapevine grafting: scion transcript profiling and defense-related metabolites induced by different rootstocks.
Organism Vitis vinifera
Experiment type Expression profiling by high throughput sequencing
Summary Genotype, climate, soil qualities, and rootstocks are among the main factors that influence grape development as well as fruit and wine composition. In this work, the rootstock/scion interactions were studied using transcriptomic and metabolic approaches on leaves of Gaglioppo variety, grafted onto thirteen different rootstocks growing in the same locations. The experiments were performed in a vineyard in the south of Italy, characterized by the Mediterranean climate with warm and dry summers. Whole leaf transcriptome of ‘Gaglioppo’ grafted on five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary (e.g. carbohydrate and transport) and secondary metabolism (e.g. phenylpropanoids pathways and response to stress) was observed. Interestingly, genes involved in the priming defense responses (e.g stilbenes and defense genes) were strongly activated in some graft combinations. These results were further confirmed by the quantification of stilbene contents. According with these observations, the leaves of ‘Gaglioppo’ grafted on 1103 Paulsen showing the lowest levels of stilbene synthase transcripts and stilbene metabolites, reveal the greater sensitivity to downy mildew in in vitro assay. This study carrying out an extensive analysis of rootstock effects to scion leaves open a useful way to unravel this complex interaction
Overall design Differential expression analysis of Gaglioppo variety grafted onto five different rootstocks
Contributor(s) Chitarra W, Perrone I, Avanzato C, Minio A, Boccacci P, Santini D, Gilardi G, Siciliano I, Gullino ML, Delledonne M, Mannini F, Gambino G
Citation(s) 28496453
Submission date Jun 06, 2016
Last update date May 15, 2019
Contact name Andrea Minio
Organization name Università di Verona
Department Applied Biotechnologies
Lab Functional Genomics Lab
Street address Via dell'Artigliere 8
City Verona
ZIP/Postal code 37129
Country Italy
Platforms (1)
GPL19359 Illumina HiSeq 1000 (Vitis vinifera)
Samples (15)
GSM2188939 Gaglioppo grafted on 1103 Paulsen, Replicate A
GSM2188940 Gaglioppo grafted on 1103 Paulsen, Replicate B
GSM2188941 Gaglioppo grafted on 1103 Paulsen, Replicate C
BioProject PRJNA325007
SRA SRP076309

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Supplementary file Size Download File type/resource
GSE82317_GEO_expression_matrix.txt.gz 1.3 Mb (ftp)(http) TXT
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