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| Status |
Public on Jun 27, 2007 |
| Title |
Dlx homeodomain transcription factor mutants (ruben-affy-mouse-313340) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The Dlx homeodomain transcription factors are implicated in regulating the function of inhibitory GABAergic interneurons; therefore understanding their functions will provide insights into disorders such as epilepsy, mental retardation and autism.
Identifying genes that are downstream of Dlx1/2 function and are relevant for the differentiation and survival of GABAergic interneurons.
During embryonic development, cortical GABAergic interneurons are generated in the proliferative zone of the medial ganglionic eminence (MGE), from where they migrate to reach their final positions in the cortex. The differentiation of these interneuron precursors is dependent on Dlx genes, as shown by Dlx1/Dlx2 double mutants, which have a block in GABAergic cell differentiation and in cell migration. When interneuron progenitors are isolated from the mutant MGE and growth in culture, they are able to proceed along their differentiation program. However, mutant cells growth in vitro show defects in cell morphogenesis and increased cell apoptosis. We hypothesize that Dlx transcription factors regulate important aspects of GABAergic neuron differentiation such as the formation and growth of axon and dendrites, and the formation of inhibitory synapses.
We generated E15.5 mouse embryos that are Dlx1/2 -/- or Dlx1/2 +/?. Genotype was confirmed by PCR. A total of 8 litters were used. For each experiment, we pooled tissue from at least 6 different embryos of the same genotype. We dissected the ventricular and subventricular zones of the MGE (rostral part). This area contains ~1 million of progenitor cells per embryo. We isolated total RNA using the Stratagene RNA Miniprep kit (these samples are called MGE+/ and MGE-/- in our proposal). In addition, we used the same area (ventricular and subventricular zones of the rostral MGE) to perform primary neuronal cultures. Cells were maintained 3 days in vitro. After that, we isolated total RNA using the Stratagene RNA Miniprep kit (samples called primary cells+/ and primary cells-/- in our proposal). We would like to perform gene expression comparison between: 1) MGE+/ and MGE-/-, and 2) primary cells+/ and primary cells-/-.
Keywords: Dlx mutants, medial ganglionic eminence, neuronal cultures
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| Contributor(s) |
Rubenstein JL |
| Citation missing |
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| Submission date |
Jun 27, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Winnie Liang |
| E-mail(s) |
wliang@tgen.org
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| Organization name |
Translational Genomics
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| Street address |
445 N. Fifth Street
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| City |
Phoenix |
| State/province |
AZ |
| ZIP/Postal code |
85012 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (5)
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| GSM206017 |
brain, forebrain: Total RNA from neuronal progenitors +/_le1 |
| GSM206018 |
brain, forebrain: Total RNA from neuronal progenitors -/-_le1 |
| GSM206019 |
brain, forebrain: Total RNA from primary neurons_le1 |
| GSM206020 |
brain, forebrain: Total RNA from primary neurons -/-_le1 |
| GSM206021 |
brain, forebrain: MGE -/- (2)_e1_le1 |
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| Relations |
| BioProject |
PRJNA101285 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8311_RAW.tar |
29.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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