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Series GSE8314 Query DataSets for GSE8314
Status Public on Jun 30, 2007
Title Characterization of weight loss and weight regain mechanisms after Roux-en-Y gastric bypass in rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Roux-en-Y gastric bypass (RYGB) is the most effective therapy for morbid obesity, but it has a ~20% failure rate. We used our established RYGB model in diet-induced obese (DIO) Sprague-Dawley rats, which reproduces human bi-phasic body weight (BW) loss pattern, to determine mechanisms contributing to success (RGYB-S) or failed (RYGB-F) RYGB. DIO rats were randomized to RYGB, sham operated Obese, and sham operated obese pair fed-linked to RYGB (PF) groups. BW, caloric intake (CI) and fecal output (FO) were recorded daily for 90 days, food efficiency (FE) was calculated, and morphologic changes were determined. Gut, adipose and thyroid hormones were measured in plasma. Mitochondrial respiratory complexes in skeletal muscle, expression of energy-related hypothalamic and fat peptides, receptors and enzymes were quantified. A 25% failure rate occurred. RYGB-S, RYGB-F and PF rats vs. Obese showed rapid BW decrease, followed by sustained BW loss in RYGB-S. RYGB-F and PF gradually increased BW. Expression profiling of both CNS (hypothalamus) and peripheral tissues (subcutaneous abdominal fat) strongly supported the involvement of a number of metabolic and feeding-related genes in the differential outcomes.
Keywords: gene expression analysis
 
Overall design 3 biological replicate RNA samples were prepared from 2 tissues (the subcutaneous abdominal fat or the hypothalamus) from rats in 3 treatment groups (rats losing weight successfully after gastric bypass surgery, rats gaining weight, and rats that were fed the same amount as the treated rats)
 
Contributor(s) Guijarro A, Suzuki S, Chen C, Kirchner H, Middleton FA, Nadtochiy S, Brookes PS, Niijima A, Inui A, Meguid MM
Citation(s) 17626126
Submission date Jun 27, 2007
Last update date Jul 31, 2017
Contact name Frank A Middleton
E-mail(s) middletf@upstate.edu
Phone 315-464-7721
Organization name SUNY Upstate Medical Univ
Department Neuroscience and Physiology
Street address 505 Irving Avenue
City Syracuse
State/province NY
ZIP/Postal code 13210
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (18)
GSM206022 Fat Pair-Fed Repl1
GSM206023 Hypothalamus Pair-Fed Repl1
GSM206024 Fat Pair-Fed Repl2
Relations
BioProject PRJNA101291

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8314_RAW.tar 43.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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