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Series GSE8320 Query DataSets for GSE8320
Status Public on Jun 27, 2008
Title Transcriptional and translational gene regulation in the maize pulvinus
Organism Zea mays
Experiment type Expression profiling by array
Summary Not much is known about the molecular processes involved during gravitropism in monocot plants such as maize. A microarray based study on the expression of genes after a gravity stimulating the maize pulvinus will provide us with valuable information and a better understanding of the underlying molecular processes involved in monocot gravitropism.
Objectives for this study included the identification of genes that were regulated at the transcriptional and translation level during gravitropism in the maize pulvinus. This was achieved by microarray analysis of total RNA versus polyribosome associated RNA during a time-course of gravity stimulation of the maize pulvinus.
Keywords: time course
Overall design Six week old maize plants were gravity stimulated by 90º reorientation. Upper (slow elongation) and lower (fast elongation) halves of the most gravity competent pulvini were harvested over a time course ranging from 2 minutes up to one hour (2min, 5 min, 15min, 30min, 60min). Pulvini samples from control (vertical, no gravity stimulation) plants were harvested and labeled as left and right. For each time point, total mRNA and polyribosome-associated mRNA were purified and the transcript profiles analyzed using Affymetrix GeneChip® Maize Genome Arrays. The experiment was repeated twice (two growing seasons) and represent two biological repetitions.
Contributor(s) Myburg H, Salinas-Mondragon R, Hammond R, Davis E, Sederoff HW
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Submission date Jun 28, 2007
Last update date Mar 17, 2012
Contact name Henrietta Myburg
Phone 919-515 2225
Organization name NC State University
Department Botany
Street address 42218 Gardner Hall
City Raleigh
State/province NC
ZIP/Postal code 27695
Country USA
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (48)
GSM206287 Left pulvinus control total RNA biorep 1
GSM206288 Upper pulvinus 2 min total RNA biorep 1
GSM206289 Upper pulvinus 5 min total RNA biorep 1
BioProject PRJNA101303

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Supplementary file Size Download File type/resource
GSE8320_RAW.tar 99.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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