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| Status |
Public on Jun 28, 2017 |
| Title |
Identification of microRNA biomarkers in the blood of breast cancer patients based on microRNA profiling |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Accumulating evidence indicates that human circulating microRNAs (miRNAs) could serve as diagnostic and prognostic biomarkers in various cancers. We aimed to explore novel miRNA biomarkers in the blood of breast cancer patients based on miRNA profiling. A miRCURY™ LNA Array was used to identify differentially altered miRNAs in the whole blood of breast cancer patients (n = 6) and healthy controls (n = 6). Levels of candidate miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in whole blood specimens of 15 breast cancer patients and 13 age-matched healthy control individuals. The miRWalk database was used to predict miRNA targets and the DAVID tool was used to identify significant enrichment pathways. A total of 171 differentially expressed miRNAs were identified by microarray, including 169 up-regulated and 2 down-regulated miRNAs in breast cancer. Five upregulated miRNAs (miR-30b-5p, miR-96-5p, miR-182-5p, miR-374b-5p, and miR-942-5p) were confirmed by qRT-PCR. The areas under the receiver operating characteristic curve of miR-30b-5p, miR-96-5p, miR-182-5p, miR-374b-5p, and miR-942-5p were 0.9333, 0.7692, 0.7590, 0.8256, and 0.8128, respectively. A total of 855 genes were predicted to be targeted by the five miRNAs, and the one cut domain family member 2 gene (ONECUT2) was a shared target of the five miRNAs. Analysis of publicly available data revealed that these dysregulated miRNAs and target genes were associated with the survival of breast cancer patients. Furthermore, the five miRNAs were significantly enriched in numerous cancer-related pathways, including “MicroRNAs in cancer”, “Pathways in cancer”, “FoxO signaling pathway”, “Ras signaling pathway”, “Rap1 signaling pathway”, “MAPK signaling pathway”, and “PI3K-Akt signaling pathway”. Our data support the potential of the five identified miRNAs as novel biomarkers for the detection of breast cancer, and indicate that they may be involved in breast cancer development and progression.
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| Overall design |
miRNA profiling in blood samples were analyzed by the 7th generation of miRCURYTM LNA Array (v.18.0, Exiqon), as previously described. It contains 3100 capture probes, covering all human, mouse and rat microRNAs annotated in miRBase 18.0, as well as all viral microRNAs related to these species.
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| Contributor(s) |
Zhang K, Wang Y |
| Citation(s) |
28359916 |
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| Submission date |
Jun 13, 2016 |
| Last update date |
Jun 30, 2017 |
| Contact name |
Ya-Wen Wang |
| E-mail(s) |
wangyawen@sdu.edu.cn
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| Organization name |
Shandong University
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| Street address |
Wen Hua Xi Road 107
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| City |
Jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
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| Platforms (1) |
| GPL22003 |
Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA325464 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83270_RAW.tar |
11.0 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE83270_normalized_matrix.txt.gz |
94.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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