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Series GSE83270 Query DataSets for GSE83270
Status Public on Jun 28, 2017
Title Identification of microRNA biomarkers in the blood of breast cancer patients based on microRNA profiling
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Accumulating evidence indicates that human circulating microRNAs (miRNAs) could serve as diagnostic and prognostic biomarkers in various cancers. We aimed to explore novel miRNA biomarkers in the blood of breast cancer patients based on miRNA profiling. A miRCURY™ LNA Array was used to identify differentially altered miRNAs in the whole blood of breast cancer patients (n = 6) and healthy controls (n = 6). Levels of candidate miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in whole blood specimens of 15 breast cancer patients and 13 age-matched healthy control individuals. The miRWalk database was used to predict miRNA targets and the DAVID tool was used to identify significant enrichment pathways. A total of 171 differentially expressed miRNAs were identified by microarray, including 169 up-regulated and 2 down-regulated miRNAs in breast cancer. Five upregulated miRNAs (miR-30b-5p, miR-96-5p, miR-182-5p, miR-374b-5p, and miR-942-5p) were confirmed by qRT-PCR. The areas under the receiver operating characteristic curve of miR-30b-5p, miR-96-5p, miR-182-5p, miR-374b-5p, and miR-942-5p were 0.9333, 0.7692, 0.7590, 0.8256, and 0.8128, respectively. A total of 855 genes were predicted to be targeted by the five miRNAs, and the one cut domain family member 2 gene (ONECUT2) was a shared target of the five miRNAs. Analysis of publicly available data revealed that these dysregulated miRNAs and target genes were associated with the survival of breast cancer patients. Furthermore, the five miRNAs were significantly enriched in numerous cancer-related pathways, including “MicroRNAs in cancer”, “Pathways in cancer”, “FoxO signaling pathway”, “Ras signaling pathway”, “Rap1 signaling pathway”, “MAPK signaling pathway”, and “PI3K-Akt signaling pathway”. Our data support the potential of the five identified miRNAs as novel biomarkers for the detection of breast cancer, and indicate that they may be involved in breast cancer development and progression.
Overall design miRNA profiling in blood samples were analyzed by the 7th generation of miRCURYTM LNA Array (v.18.0, Exiqon), as previously described. It contains 3100 capture probes, covering all human, mouse and rat microRNAs annotated in miRBase 18.0, as well as all viral microRNAs related to these species.
Contributor(s) Zhang K, Wang Y
Citation(s) 28359916
Submission date Jun 13, 2016
Last update date Jun 30, 2017
Contact name Ya-Wen Wang
Organization name Shandong University
Street address Wen Hua Xi Road 107
City Jinan
ZIP/Postal code 250012
Country China
Platforms (1)
GPL22003 Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18]
Samples (12)
GSM2197916 2K001
GSM2197917 2K002
GSM2197918 2K003
BioProject PRJNA325464

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83270_RAW.tar 11.0 Mb (http)(custom) TAR (of GPR)
GSE83270_normalized_matrix.txt.gz 94.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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