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Status |
Public on Sep 01, 2016 |
Title |
Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
ZFAT is a nuclear protein that harbors putative DNA-binding domains. Studies in mice have shown that ZFAT plays a critical role in survival and differentiation in T cells. However, molecular mechanisms whereby ZFAT regulates gene expression programs in T cells remain poorly unknown. We here identify the direct target genes of ZFAT in thymocytes through combination of chromatin immunoprecipitation with sequencing and transcriptional profiling.
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Overall design |
ChIPseq data were obtained for a zinc-finger transcription factor, ZFAT, and histone modifications (H3K9me3 and H3K9ac/K27ac) to further elucidate the functions of ZFAT in thymocytes.
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Contributor(s) |
Ishikura S, Tsunoda T, Nakabayashi K, Shirasawa S |
Citation(s) |
27591365 |
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Submission date |
Jun 15, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Kazuhiko Nakabayashi |
E-mail(s) |
nakabaya-k@ncchd.go.jp
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Organization name |
National Research Institute for Child Health and Development
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Department |
Department of Maternal-Fetal Biology
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Street address |
2-10-1 Okura
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City |
Setagaya |
State/province |
Tokyo |
ZIP/Postal code |
157-8535 |
Country |
Japan |
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Platforms (1) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA325737 |
SRA |
SRP076595 |