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| Status |
Public on Sep 01, 2016 |
| Title |
Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ZFAT is a nuclear protein that harbors putative DNA-binding domains. Studies in mice have shown that ZFAT plays a critical role in survival and differentiation in T cells. However, molecular mechanisms whereby ZFAT regulates gene expression programs in T cells remain poorly unknown. We here identify the direct target genes of ZFAT in thymocytes through combination of chromatin immunoprecipitation with sequencing and transcriptional profiling.
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| Overall design |
ChIPseq data were obtained for a zinc-finger transcription factor, ZFAT, and histone modifications (H3K9me3 and H3K9ac/K27ac) to further elucidate the functions of ZFAT in thymocytes.
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| Contributor(s) |
Ishikura S, Tsunoda T, Nakabayashi K, Shirasawa S |
| Citation(s) |
27591365 |
| |
| Submission date |
Jun 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kazuhiko Nakabayashi |
| E-mail(s) |
nakabaya-k@ncchd.go.jp
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| Organization name |
National Research Institute for Child Health and Development
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| Department |
Department of Maternal-Fetal Biology
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| Street address |
2-10-1 Okura
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| City |
Setagaya |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA325737 |
| SRA |
SRP076595 |
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