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| Status |
Public on Jun 20, 2018 |
| Title |
MiR-200a Regulates CDK4/6 Inhibitor Effect by Targeting CDK6 in Metastatic Melanoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Three metastatic melanoma cell lines (BD-0548-ME, DP-0574-ME and WP-0614-ME) were transfected with miR precursors: hsa-miR negative control (NC) or hsa-miR-200a-3p precursors (miR-200a). The objective of this experiment was to determine miR-200a targets in metastatic melanoma cell lines. We selected low -miR-200a expression cell lines and then we overexpressed miR-200a or NC. Finally, we compared the metastatic melanoma cell lines with miR-200a overexpression (miR-200a) to the same cell line transfected with the negative control miR (NC).
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| |
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| Overall design |
Total RNA was extracted from three melanoma cell lines and RNA-Seq by using Illumina HiSeq 2500.
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| |
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| Contributor(s) |
Bustos MA, Ono S, Marzese DM, Salomon MP, Hsu S, Nelson N, Hoon DS |
| Citation(s) |
28526299 |
| |
| Submission date |
Jun 20, 2016 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Dave S.B. Hoon |
| E-mail(s) |
hoond@jwci.org
|
| Organization name |
Saint John's Cancer Institute
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| Department |
Translational Molecular Medicine
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| Street address |
2200 Santa Monica Boulevard
|
| City |
Santa Monica |
| State/province |
CA |
| ZIP/Postal code |
90403 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA326229 |
| SRA |
SRP076790 |
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