|
| Status |
Public on Aug 25, 2016 |
| Title |
CLIP-seq analysis of integrase-RNA interactions |
| Organism |
Human immunodeficiency virus 1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Integrase CLIP-seq experiments were conducted on wild-type and eccentric HIV-1 virions generated in the presence of allosteric integrase inhibitors and IN K264/266A and R269/K273A mutations
|
| |
|
| Overall design |
Integrase CLIP-seq experiments were conducted by immunoprecipitation of integrase-RNA complexes from fully formed mature and eccentric virus particles. Libraries of RNA molecules bound by integrase were generated and sequenced by Illumina Hi-Seq2000 and 2500 platforms.
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| |
|
| Contributor(s) |
Kutluay SB |
| Citation(s) |
27565348 |
| |
| Submission date |
Jun 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sebla Bulent Kutluay |
| E-mail(s) |
kutluay@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Street address |
Washington University School of Medicine
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL20319 |
Illumina HiSeq 2000 (Human immunodeficiency virus 1) |
| GPL22068 |
Illumina HiSeq 2500 (Human immunodeficiency virus 1) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA326435 |
| SRA |
SRP076874 |
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