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| Status |
Public on Feb 24, 2017 |
| Title |
ASXL1 Interacts with the Cohesin Complex to Maintain Chromatid Separation and Gene Expression for Normal Hematopoiesis [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ASXL1 is frequently mutated in a spectrum of myeloid malignancies with poor prognosis. Loss of Asxl1 leads to myelodysplastic syndrome-like disease in mice, however, the underlying molecular mechanisms remain unclear. Here, we report that ASXL1 interacts with the cohesin complex, which has been shown to guide sister chromotid segregation and to regulate gene expression. Loss of Asxl1 impairs the cohesin function as reflected by an impaired telophase chromatid disjunction in hematopoietic cells. ChIP-seq data revealed that ASXL1, RAD21 and SMC1A share 93% of genomic binding sites at promoter regions in lineage-cKit+ (LK) cells. We have showed that loss of Asxl1 reduced the genome binding of RAD21 and SMC1A, and altered the expression of ASXL1/cohesin target genes in LK cells. Our study underscores the ASXL1-cohesin interaction as a novel means to maintain normal sister chromatid separation and to regulate gene expression in hematopoietic cells.
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| |
|
| Overall design |
The DEG genes' relation with the changes of ASXL1 peaks and Cohesin peaks changes
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| |
|
| Contributor(s) |
Li Z, Zhang P, Yang F |
| Citation(s) |
28116354 |
| Submission date |
Jul 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Zhang |
| E-mail(s) |
pxz36@miami.edu
|
| Organization name |
University of Miami Miller School of Medicine
|
| Lab |
Feng-Chun Yang
|
| Street address |
1011 NW 15th Street
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE83962 |
ASXL1 Interacts with the Cohesin Complex to Maintain Chromatid Separation and Gene Expression for Normal Hematopoiesis |
|
| Relations |
| BioProject |
PRJNA327562 |
| SRA |
SRP077725 |
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