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| Status |
Public on Dec 25, 2018 |
| Title |
HIF-1α activation is sufficient for the development of MDS |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hypoxia inducible factor-1α (HIF-1α) is a critical transcription factor for the hypoxic response, angiogenesis, normal hematopoietic stem cell regulation, and cancer development. Importantly, HIF-1α is also a key regulator for immune cell activation. In order to determine whether HIF-1α is sufficient for developing MDS phenotypes, we generated blood specific inducible HIF-1α transgenic mice. Using Vav1-Cre/Rosa26-loxP-Stop-loxP (LSL) rtTA driver, stable HIF-1α can be induced in a doxycycline administration dependent manner. After induction, HIF-1α-induced mice developed thrombocytopenia, leukocytopenia, macrocytic anemia, and multi-lineage dysplasia. We also found activation of both innate and adaptive immunity in HIF-1α- induced mice compared to those from control mice. Taken together, these data suggest that HIF-1α is sufficient to trigger a variety of key MDS features
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| Overall design |
Expression profiles of mRNA in HSPCs from constitutively active form of HIF1a protein induced mice and their control mice.
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| Contributor(s) |
Hayashi Y, Huang G |
| Citation(s) |
30139811 |
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| Submission date |
Jul 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yoshihiro Hayashi |
| Organization name |
Tokyo University of Pharmacy and Life Sciences
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| Lab |
Laboratory of Oncology
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| Street address |
1432-1 Horinouchi
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| City |
Hachioji |
| State/province |
Tokyo |
| ZIP/Postal code |
1920392 |
| Country |
Japan |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA327751 |
| SRA |
SRP077870 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83988_countsTable.xls.gz |
378.3 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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