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Series GSE8404 Query DataSets for GSE8404
Status Public on Apr 07, 2008
Title Influence of Water Chemistry on Toxicogenomics of Chronic Lead Toxicity to the Fathead Minnow (Pimephales promelas)
Organism Pimephales promelas
Experiment type Expression profiling by array
Summary Despite major successes in reducing the risks of lead (Pb) exposure over the past few decades, two issues of considerable importance remain unresolved: (1) how differences in water chemistry influence acute and chronic Pb toxicity, and (2) the elucidation of specific toxic mechanisms and modes of action (MOA). To more clearly define the water chemistry parameters mediating Pb toxicity we evaluated the effects of hardness (as CaSO4) and DOC (as humic acid (HA)) during chronic (150d) exposures to the fathead minnow (Pimephales promelas). Traditional toxicological endpoints were examined alongside gene expression analyses to help clarify the underlying mechanisms and MOA of Pb toxicity and to identify robust molecular markers of exposure and effect.
Keywords: time course, chronic lead (Pb) exposure
 
Overall design To analyze the gene expression responses to low-level Pb exposures fish were exposed +/- Pb in low ionic strength base water and fish collected at 2d, 4d, 10d, and 30d for microarray analysis. Equal amounts of RNA from all no lead controls were pooled (18 fish total for 2d & 4d exposures; 12 fish total for 10d & 30d exposures) as reference samples for hybridization with each of 3 separate biological replicate pools (6 fish total for 2d & 4d exposures; 4 fish total for 10d & 30d exposures) of RNA from age-matched, lead-exposed fish. Each pair of hybridizations was repeated with dye-swaps. Additional repeats were performed for various arrays to ensure quality of data obtained from initial hybridization.
 
Contributor(s) Mager EM, Wintz H, Vulpe CD, Brix KV, Grosell M
Citation(s) 18346799
Submission date Jul 06, 2007
Last update date Mar 17, 2012
Contact name Edward Mager
E-mail(s) emager@rsmas.miami.edu
Organization name University of Miami, RSMAS
Department MBF
Lab Martin Grosell
Street address 4600 Rickenbacker Cswy.
City Miami
State/province FL
ZIP/Postal code 33149
Country USA
 
Platforms (1)
GPL5443 Vulpe Lab UC-Berkeley Fathead Minnow v1.0
Samples (30)
GSM208394 2d lead-exposed (replicate A) vs. non-exposed fathead minnows
GSM208396 2d lead-exposed (replicate A) vs. non-exposed fathead minnows (dye swap)
GSM208398 2d lead-exposed (replicate B) vs. non-exposed fathead minnows
Relations
BioProject PRJNA101459

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8404_RAW.tar 21.0 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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