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Series GSE84129 Query DataSets for GSE84129
Status Public on Jan 01, 2023
Title CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [G1ER ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Hematopoietic stem and progenitor cell (HSPC) formation and lineage differentiation involve gene expression programs orchestrated by transcription factors and epigenetic regulators. Knockdown of the chromatin remodeler chromodomain-helicase-DNA-binding protein 7 (CHD7) expanded phenotypic HSPCs, erythroid, and myeloid lineages in zebrafish and mouse embryos. CHD7 acts to suppress hematopoietic differentiation in a cell autonomous manner in the embryo and adult. CHD7 chromatin immunoprecipitation in human CD34+ and mouse HSPCs revealed enrichment of binding motifs for hematopoietic transcription factors including Runx1 and GATA factors, and decreased Runx1 occupancy correlated with loss of CHD7 occupancy. CHD7 physically interacts with Runx1 and suppresses Runx1-induced expansion of HSPCs during development, providing both physical and genetic evidence for the Runx1-CHD7 interaction. CHD7 modulates Runx1 activity to provide proper timing and function of HSPCs as they emerge during hematopoietic development or mature in adults, representing a distinct and evolutionarily conserved control mechanism to ensure accurate hematopoietic lineage differentiation.
 
Overall design ChIP sequencing of GATA2, RUNX1 and PolII-phospho-serineII in G1ER cells.
 
Contributor(s) Choudhouri A, Superdock M, Yang S
Citation(s) 32883883
Submission date Jul 07, 2016
Last update date Apr 03, 2023
Contact name Leonard Zon
E-mail(s) zon@enders.tch.harvard.edu
Organization name Boston Children's Hospital
Department Oncology/Hematology
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (14)
GSM2227574 G1ER_WT_GATA2
GSM2227575 G1ER_WT_RUNX1
GSM2227576 G1ER_CHD7-KO_GATA2
This SubSeries is part of SuperSeries:
GSE84131 CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation
Relations
BioProject PRJNA328126
SRA SRP078052

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84129_AC3_GRCm38_peaks.narrowPeak.gz 3.9 Mb (ftp)(http) NARROWPEAK
GSE84129_AC4_GRCm38_peaks.narrowPeak.gz 3.9 Mb (ftp)(http) NARROWPEAK
GSE84129_RAW.tar 5.4 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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