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| Status |
Public on Jan 13, 2017 |
| Title |
On the Relationships in Rhesus Macaques between Chronic Ethanol Consumption and the Brain Transcriptome (Central Nucleus of the Amigdala - CeA) |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This is the first description of the effects of chronic ethanol consumption on the brain transcriptome in a non-human primate (rhesus macaque). Thirty male animals self-administered ethanol on a daily basis for over 12 months. Gene expression was measured with RNA-Seq in the central nucleus of the amygdala (CeA). Genes negatively correlated with consumption were enriched in functional annotations associated with translation and included clusters of ribosomal and mitochondrial ribosomal proteins. Genes positively correlated with consumption were enriched in membrane annotations including neuron and synapse part. Genes in the latter category included Ctnna2, Shank1, Vamp2, and Syn1. Gene-level expression data were clustered using the Weighted Gene Coexpression Network Analysis (WGCNA) algorithm to identify hub nodes affected by excessive consumption. Key hubs included Nf1, Nkain2, Tmem108 and Penk. The data were also analyzed for alternative exon usage (Iancu et al., 2015). Additional translation and membrane related genes affected by excessive consumption were detected. These included Grm2, Dpysl2 and Kcnc4. Overall, the data illustrate that the effects of excessive ethanol consumption are broad, consistent with the observation that ethanol affects the expression of numerous hub genes.
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| Overall design |
28 samples of Rhesus Macaques that were experimentally naïve at the onset of alcohol induction (Macaca mulatta, n = 28, 4-11 years, 6-8 kg), were first induced to drink 4% (w/v) ethanol and then allowed to self-administer ethanol for over 12 months under open access conditions (22h/d, 7d/wk) with concurrent meals and continuous access to water.
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| Contributor(s) |
Iancu OD, Colville A, Walter NA, Darakjian P, Oberbeck D, Daunais JB, Zheng CL, Searles RP, McWeeney S, Grant KA, Hitzemann R |
| Citation(s) |
28247455 |
| |
| Submission date |
Jul 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Priscila Darakjian |
| E-mail(s) |
darakjia@ohsu.edu
|
| Organization name |
Oregon Health and Science University
|
| Department |
Behavioral Neuroscience
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| Street address |
3181 SW Sam Jackson Park Road, L470
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
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| Platforms (1) |
| GPL14954 |
Illumina HiSeq 2000 (Macaca mulatta) |
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| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE96732 |
On the Relationships in Rhesus Macaques between Chronic Ethanol Consumption and the Brain Transcriptome |
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| Relations |
| BioProject |
PRJNA328925 |
| SRA |
SRP078410 |
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