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Series GSE84541 Query DataSets for GSE84541
Status Public on Jul 20, 2016
Title Trancriptomic analysis of PKA and PKC signalling gene cascade in cultured human ovarian granulosa cells (KGN) [FSK treatments]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The oocyte’s capacity to complete maturation, to succeed fertilization and to reach the blastocyst stage is what defines the oocyte’s competence. The oocyte acquires this competence working closely with somatic cells of the follicle. Cumulus and granulosa cells provided support for the oocyte’s development and conversely the oocyte influence follicular cell growth and differentiation. Existing studies support the idea that follicular-stimulated hormone and luteinizing hormone play an essential role in oocyte competence acquisition through protein kinase A (PKA) and protein kinase C (PKC) signalling in granulosa cells. Therefore, human-like granulosa cells (KGN) were treated with forskolin 10 μM and phorbol 12-myristate 13-acetate 0.1 μM for 24 hours in order to process a transcriptomic analysis of differentially express genes between treatment. Over 2000 genes were founded to be differentially express at cut-off fold change of 1.5 and a p-value of 0.05. Five major upstreams, EGF, TGFB1, VEGF, FGF2 and HGF were founded to play an important role in competence acquisition thought PKA and PKC signalling. Differentially expressed targeted genes of both signalling pathways were classified in seven major ovarian functions such as PTGS2, IL8 and IL6 in inflammation, STAR, CYP11A1, CYP19A1 in steroidogenesis, VEGFC, VEGFA, CXCR4 in angiogenesis, AREG, EGFR, SPRY2 in differentiation, BAX, BCL2L12, CASP1 in apoptosis, CCND1, CCNB1, CCNB2 in division and MMP1, MMP9, TIMP1 in ovulation. Taken together, the results of this study suggest that PKA and PKC signalling potentiate their effects in some functions such as inflammation and apoptosis while some others are more specific to one or the other protein kinase like differentiation, ovulation and angiogenesis that are thought to be more PKC-dependent in human granulosa cells.
 
Overall design 8 samples were analysed, 4 controls compared to 4 Forskolin treatments (total of 4 replicates).
 
Contributor(s) Tremblay PG, Dufort I, Sirard M
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Submission date Jul 19, 2016
Last update date Jun 30, 2017
Contact name Patricia G Tremblay
E-mail(s) patricia.tremblay.6@ulaval.ca
Organization name Laval University
Department Sciences animales
Lab Chaire de Recherche du Canada en génomique appliquée à la reproduction
Street address 2440 Hochelaga Blvd
City Québec
State/province Québec
ZIP/Postal code G1V 0A6
Country Canada
 
Platforms (1)
GPL10332 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version)
Samples (4)
GSM2241703 FSK_24H_rep1
GSM2241704 FSK_24H_rep2
GSM2241705 FSK_24H_rep3
This SubSeries is part of SuperSeries:
GSE84543 Trancriptomic analysis of PKA and PKC signalling gene cascade in cultured human ovarian granulosa cells (KGN)
Relations
BioProject PRJNA329592

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84541_RAW.tar 14.8 Mb (http)(custom) TAR (of TXT)
GSE84541_norm_log2_ratios_FSK.txt.gz 1.2 Mb (ftp)(http) TXT
Processed data are available on Series record

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