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| Status |
Public on Jan 01, 2017 |
| Title |
Small RNA sequencing of large dense-core vesicle in bovine chromaffin cells |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Objectives: MicroRNA (miRNA) can be released to the extracellular medium and participates in neuronal communication. We investigate the mechanisms of miRNA exocytosis by vesicle fusion as a neuromodulator in a manner that are disparate from silencing gene expression.
Methods: Small RNA sequencing data of large dense-core vesicle were generated by next-generation sequencing (NGS) in triplicate using Illumina Hiseq 2500.
Results: Large dense-core vesicles contain a variety of known and novel miRNAs inside including miR-375.
Conclusion: miRNAs can be novel neuromodulators, which are stored in LDCVs and released by vesicle fusion by SNARE assembly and synaptotagmin-1
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| |
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| Overall design |
Small RNA sequencing of large dense-core vesicle in bovine chromaffin cells from triplicate including technical replicate and biological replicate.
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| |
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| Contributor(s) |
Park Y, Karakülah G |
| Citation(s) |
28358390 |
| |
| Submission date |
Jul 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yongsoo Park |
| E-mail(s) |
yongsoo.park@deu.edu.tr
|
| Phone |
+90 232 26567
|
| Organization name |
Izmir International Biomedicine and Genome Institute (iBG-izmir)
|
| Street address |
Balcova
|
| City |
Izmir |
| ZIP/Postal code |
35340 |
| Country |
Turkey |
| |
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| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA335347 |
| SRA |
SRP079882 |
