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Series GSE8492 Query DataSets for GSE8492
Status Public on Feb 22, 2008
Title Analysis of effect of sirt1 deficiency on global gene expression in testis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Testis were obtained from 2 sibling pairs of of young male mice, 1 knock-out and 1 heterozygous for the Sirt1 allele, and used to compare global gene expression using spotted oligo microarrays (the MEEBO arrays from the Stanford microarray core- MOE series). Eighty genes were up or down regulated, where the criteria for differential expression was a greater than 2 fold change in expression level on at least 5 of the 6 sample arrays. The list of differentially expressed genes was enriched for genes involved in spermatogenesis.
Keywords: spotted oligonucleotide microarray, mouse
 
Overall design 2 pairs of Sirt1 knock-out and heterozygous testis were analyzed. The global gene expression was assessed intriplicate for each pair, including 1 dye swap.
 
Contributor(s) Allsopp R, Coussens M, Maresh G
Citation(s) 18270565
Submission date Jul 16, 2007
Last update date Jan 18, 2013
Contact name rich allsopp
E-mail(s) allsopp@hawaii.edu
Phone 808 692-1412
Organization name university of hawaii
Department anatomy & reprod biol
Street address 651 Ilalo St
City Honolulu
State/province HI
ZIP/Postal code 96813
Country USA
 
Platforms (1)
GPL5467 MEEBO Mus musculus 39k spotted oligo array (MOE series)
Samples (6)
GSM210104 first analysis of pair #1 Sirt1 knock-out versus control testis
GSM210201 cDNA spotted array, Sirt1 KO/Het pair #1, replicate 3 dye swap
GSM210202 cDNA spotted array, Sirt1 KO/Het pair #1, replicate 2
Relations
BioProject PRJNA101589

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
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Supplementary data files not provided
Processed data included within Sample table

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