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Status |
Public on Feb 22, 2008 |
Title |
Analysis of effect of sirt1 deficiency on global gene expression in testis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Testis were obtained from 2 sibling pairs of of young male mice, 1 knock-out and 1 heterozygous for the Sirt1 allele, and used to compare global gene expression using spotted oligo microarrays (the MEEBO arrays from the Stanford microarray core- MOE series). Eighty genes were up or down regulated, where the criteria for differential expression was a greater than 2 fold change in expression level on at least 5 of the 6 sample arrays. The list of differentially expressed genes was enriched for genes involved in spermatogenesis. Keywords: spotted oligonucleotide microarray, mouse
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Overall design |
2 pairs of Sirt1 knock-out and heterozygous testis were analyzed. The global gene expression was assessed intriplicate for each pair, including 1 dye swap.
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Contributor(s) |
Allsopp R, Coussens M, Maresh G |
Citation(s) |
18270565 |
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Submission date |
Jul 16, 2007 |
Last update date |
Jan 18, 2013 |
Contact name |
rich allsopp |
E-mail(s) |
allsopp@hawaii.edu
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Phone |
808 692-1412
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Organization name |
university of hawaii
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Department |
anatomy & reprod biol
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Street address |
651 Ilalo St
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City |
Honolulu |
State/province |
HI |
ZIP/Postal code |
96813 |
Country |
USA |
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Platforms (1) |
GPL5467 |
MEEBO Mus musculus 39k spotted oligo array (MOE series) |
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Samples (6)
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GSM210104 |
first analysis of pair #1 Sirt1 knock-out versus control testis |
GSM210201 |
cDNA spotted array, Sirt1 KO/Het pair #1, replicate 3 dye swap |
GSM210202 |
cDNA spotted array, Sirt1 KO/Het pair #1, replicate 2 |
GSM210211 |
first analysis of pair #2 Sirt1 knock-out versus control testis |
GSM210287 |
replicate #2 of pair #2 Sirt1 knock-out versus control testis |
GSM210288 |
replicate #3 of pair #2 Sirt1 knock-out versus control testis dye swap |
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Relations |
BioProject |
PRJNA101589 |