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| Status |
Public on Jun 30, 2018 |
| Title |
Transcription start site positioning is not affected by +1 nucleosome depletion |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The relationship between the positioning of nucleosomes and transcription start sites (TSSs) is unclear. Here, we report that deletion of the Fun30 class chromatin remodelers Fft2 and Fft3 leads to depletion of +1 nucleosome occupancy. Strikingly, in the vast majority of cases, this depletion had no impact on the placement of the TSS, favoring a model where TSS placement is primarily DNA sequence-driven.
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| Overall design |
5'-Cap Analysis of Gene Expression (CAGE) of S. pombe wild type and fft2fft3 double mutant cells, in triplicates.
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| Contributor(s) |
Thieffry A, Steglich B, Persson J, Boyd M, Bornholdt J, Prasad P, Nielsen O, Ekwall K, Sandelin A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Axel Thieffry |
| E-mail(s) |
axel.thieffry@gmail.com
|
| Organization name |
BioPhero-FMC
|
| Department |
R&D
|
| Street address |
Lersø Parkallé 42-44
|
| City |
Copenhagen |
| State/province |
Hovedstaden |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
| |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA336018 |
| SRA |
SRP080753 |
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