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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 02, 2016 |
| Title |
The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Neutrophils, eosinophils and “classical” monocytes collectively account for ~70% of human blood leukocytes and are among the shortest-lived cells in the body. Precise regulation of the lifespan of these myeloid cells is critical to maintain protective immune responses while minimizing the deleterious consequences of prolonged inflammation. However, how the lifespan of these cells is strictly controlled remains largely unknown. Here, we identify a novel long non-coding RNA (lncRNA) that we termed Morrbid, which tightly controls the survival of neutrophils, eosinophils and “classical” monocytes in response to pro-survival cytokines. To control the lifespan of these cells, Morrbid regulates the transcription of its neighboring pro-apoptotic gene, Bcl2l11 (Bim), by promoting the enrichment of the PRC2 complex at the Bcl2l11 promoter to maintain this gene in a poised state. Notably, Morrbid regulates this process in cis, enabling allele-specific control of Bcl2l11 transcription. Thus, in these highly inflammatory cells, changes in Morrbid levels provide a locus-specific regulatory mechanism that allows for rapid control of apoptosis in response to extracellular pro-survival signals. As MORRBID is also present in humans and dysregulated in patients with hypereosinophilic syndrome, this lncRNA may represent a potential therapeutic target for inflammatory disorders characterized by aberrant short-lived myeloid cell lifespan.
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| Overall design |
Examination of 4 different histone modifications in mouse neutrophils.
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| Contributor(s) |
Kotzin JJ, Spencer SP, McCright SJ, Uthaya Kumar DB, Collet M, Mowel WK, Elliott EN, Uyar A, Makiya MA, Dunagin MC, Harman CD, Virtue AT, Zhu S, Bailis W, Stein J, Hughes C, Raj A, Wherry EJ, Goff LA, Klion AD, Rinn JL, Williams A, Flavell RA, Henao-Mejia J |
| Citation(s) |
27525555 |
| Submission date |
Aug 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Asli Uyar |
| E-mail(s) |
asli.uyar@jax.org
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| Organization name |
The Jackson Laboratory
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| Department |
Computational Sciences
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| Street address |
10 Discovery Dr
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| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06032 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE85073 |
The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan |
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| Relations |
| BioProject |
PRJNA336059 |
| SRA |
SRP080773 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85072_ChIPSeq_consensusPeaks_K27ac_normalizedReadCounts.xls.gz |
3.2 Mb |
(ftp)(http) |
XLS |
| GSE85072_ChIPSeq_consensusPeaks_K36me3_normalizedReadCounts.xls.gz |
2.4 Mb |
(ftp)(http) |
XLS |
| GSE85072_ChIPSeq_consensusPeaks_K4me1_normalizedReadCounts.xls.gz |
3.9 Mb |
(ftp)(http) |
XLS |
| GSE85072_ChIPSeq_consensusPeaks_K4me3_normalizedReadCounts.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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