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| Status |
Public on Oct 24, 2016 |
| Title |
In-phase oscillation of global regulons is orchestrated by a pole-specific organizer |
| Organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Cell fate determination in the asymmetric bacterium Caulobacter crescentus (Caulobacter) is triggered by the localization of the developmental regulator SpmX to the old (stalked) cell pole during the G1-S transition. While SpmX is required to localize and activate the cell fate-determining kinase DivJ at the stalked pole in Caulobacter, it is also required for organelle (stalk) positioning in the cousin Asticaccaulis. We unearth the conserved s54-dependent transcriptional activator regulator TacA as global regulator in Caulobacter whose activation by phosphorylation is indirectly down-regulated by SpmX. Using a combination of forward genetics and cytological screening we uncover a previously uncharacterized and polarized component (SpmY) of the TacA phosphorylation control system, and we show that the SpmY function and localization is conserved. Thus, we demonstrate that SpmX organizes a site-specific, ancestral and multi-functional regulatory hub whose function includes the coordinated control of two co-oscillating global transcriptional regulators, CtrA and TacA.
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| |
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| Overall design |
Examination of 2 transcription factors occupancy in WT and two mutant backgrounds + assessment of synthetic lethal and viable genes in WT and one null-allele background
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| |
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| Contributor(s) |
Janakiraman B, Mignolet J, Narayanan S, Viollier PH, Radhakrishnan SK |
| Citation(s) |
27791133 |
| |
| Submission date |
Aug 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Johann Mignolet |
| E-mail(s) |
johann.mignolet@unil.Ch
|
| Organization name |
UNIL
|
| Department |
DMF
|
| Lab |
Veening lab
|
| Street address |
Biophore Quartier UNIL-SORGE
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL21317 |
Illumina HiSeq 2500 (Caulobacter crescentus NA1000) |
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| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA336492 |
| SRA |
SRP080925 |
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