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Series GSE8522 Query DataSets for GSE8522
Status Public on Feb 29, 2008
Title RNA from 3 d and 2 d old embryos of mibta52b, mibm132 and mibtfi91, and RNA from 3 d, 2d and 1 d old embryos of mibta52b
Organism Danio rerio
Experiment type Expression profiling by array
Summary The Notch signaling is an evolutionarily conserved signal transduction cascade that plays essential roles in a variety of developmental processes. In this study, we examined the mib mutants for defects in pancreas development using in situ hybridization and GFP expression analysis of pancreas-specific GFP lines, and carried out the global gene expression profile analysis of three different mib mutantalleles. Global expression profile analysis showed that there is a significant difference in gene expression profile of wild type and three mib mutant alleles. There are 91 differentially expressed genes that are common to all three mib alleles. Through detailed analysis of microarray data we have identified several previously characterized genes and some putative Notch-responsive genes involved in pancreas development, and validated theirexpression profile using real-time PCR and in situ hybridization. We propose that this study provides a useful resource of global gene expressionprofile in mib mutants, which will be helpful in identifying the function of novel genes involved in Notch signaling and Notch-responsive genesinvolved in a variety of developmental processes.
Keywords: comparison of global gene expression in mib mutant and wild type embryos
 
Overall design All samples were analysed on Zebrafish microarray chips generated at the Genome Institute of Singapore
 
Contributor(s) Hegde A, Tay KQ, Qiu N, Govindarajan KR, George J, Ho SH, Gong Z, Mathavan S, Jiang Y
Citation(s) 18213387
Submission date Jul 19, 2007
Last update date Mar 17, 2012
Contact name Govindarajan Kunde Ramamoorthy
E-mail(s) mathavans@gis.a-star.edu.sg
Phone +65-64788059
Fax +65-64789058
Organization name Genome Institute of Singapore
Department Research Computing Group
Street address 60, Biopolis Street, Genome
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platforms (1)
GPL2715 Zf_16.5K_oligo_COMP_v3Apx
Samples (42)
GSM211578 mibm132 72h (AH_WT72hVsm13272h_BRI_TR2_39)
GSM211579 mibm132 72h (AH_WT72hVsm13272h_BRI_TR3_40)
GSM211580 mibm132 72h (AH_WT72hVsm13272h_BRII_TR2_52)
Relations
BioProject PRJNA101645

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Supplementary data files not provided
Processed data included within Sample table
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