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| Status |
Public on Aug 08, 2019 |
| Title |
The luh-4 mutant has a differential transcriptional program in response to Al stress |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate how LUH regulates Al binding to cell-wall and thus inhibition of root growth under Al stress, a transcriptional analysis through RNAseq was performed by comparing the luh-4 mutant and WT plants in the presence or absence of Al. In WT plants, 316 and 336 genes, respectively, were up- and down-regulated at least 2-fold, while in luh-4, 239 and 305 genes, respectively, were up- and down-regulated at least 2-fold by Al stress. By comparison with WT seedlings, we found that 103 and 38 genes, respectively, were up- and down-regulated at least 2-fold in the non-stressed luh-4 mutant, while 77 and 33 genes, respectively, were up- and down-regulated at least 2-fold in Al-subjected luh-4 roots.
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| Overall design |
Total RNA obtained from control (non-treated roots) and Al-exposed roots. Six day old plants were treated with and without Al treatment for 4 h, then total RNA was isolated using the RNeasy® Plant Mini Kit (QIAGEN), and then Illumina sequencing was performed.
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| Contributor(s) |
Yang Z, Geng X, Ding Z |
| Citation missing |
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| Submission date |
Aug 08, 2016 |
| Last update date |
Aug 09, 2019 |
| Contact name |
Zhong-Bao Yang |
| E-mail(s) |
zbyang@sdu.edu.cn
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| Organization name |
Shandong University (Qingdao)
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| Department |
School of Life Science
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| Lab |
he Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education
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| Street address |
72# Binhai Road
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| City |
Qingdao |
| State/province |
Shandong |
| ZIP/Postal code |
266237 |
| Country |
China |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA338114 |
| SRA |
SRP081071 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85292_RAW.tar |
7.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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