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| Status |
Public on Aug 08, 2016 |
| Title |
Mesenchymal stem cell-derived exosomes stimulate cycling quiescence and early breast cancer dormancy in bone marrow |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Transwell cultures were established in 6-well plates with 0.4 micron inserts. Equal amounts (6x10e4) of BCCs and MSCs were added to the outer and inner wells, respectively, in DMEM with 10% exosome-free FCS. After 24 h, the inner wells with BCCs were discarded. The MSCs were washed with PBS and then incubated with DMEM with 2% exosome-free FCS. At 48 h, exosomes were isolated from the MSC media. The exosomes from triplicate wells were added to duplicate naïve BCCs at 2.5x105/well of 6-well plates.
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| Overall design |
qPCR miRNA expression profiling. RNA from MSCs-Exosomes were analyzed as indicated in the summary. The PCR was run on the 7300 Real-Time PCR System using the following thermal-cycling parameters: 94 0C for 15 min, 40 cycles at 940 C for 10 sec, 550 C for 30s, 700 C for 30 sec followed by a melting curve analysis.
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| Contributor(s) |
Guiro K, Sandiford OA |
| Citation(s) |
27569215 |
| |
| Submission date |
Aug 08, 2016 |
| Last update date |
Nov 28, 2016 |
| Contact name |
Pranela Rameshwar |
| E-mail(s) |
rameshwa@njms.rutgers.edu
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| Organization name |
Rutgers NJ Medical School
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| Department |
Medicine
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| Lab |
Stem Cell and Translational Medicine
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| Street address |
185 South Orange Avenue
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| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
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| Platforms (1) |
| GPL22300 |
Qiagen Human miFinder miScript miRNA PCR panel (MIHS-001Z) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA338200 |
